methylcellulose and Necrosis

A thermo-sensitive methylcellulose (MC) hydrogel containing silver oxide nanoparticles (NPs) was prepared via one-pot synthesis in which a silver acetate precursor salt (CH

Topics: Acetates; Animals; Anti-Bacterial Agents; Burns; Drug Liberation; Hydrogels; Inflammation; Male; Methylcellulose; Nanoparticles; Necrosis; Oxides; Porosity; Rats, Sprague-Dawley; Silver Compounds; Wound Healing

Developing effective techniques for the cryopreservation of human adipose-derived adult stem cells (ASCs) could increase the usefulness of these cells in tissue engineering and regenerative medicine. To this end, we investigated the post-freeze/thaw viability and apoptotic behavior of Passage 1 (P1) adult stem cells (ASCs) in 11 different media: (i) the traditional media containing Dulbecco's modified Eagle's medium (DMEM) with 80% fetal calf serum (FCS) and 10% dimethyl sulfoxide (DMSO), (ii) DMEM with 80% human serum (HS) and 10% DMSO, (iii) DMEM with 1% methyl cellulose (MC) and 10% of either HS or FCS or DMSO, and (iv) DMEM with 0%, 2%, 4%, 6%, 8%, or 10% DMSO. Approximately 1 mL (10(6) cells/mL) of P1 ASCs were frozen overnight in a -80 degrees C freezer and stored in liquid nitrogen for 2 weeks before being rapidly thawed in a 37 degrees C water bath (1-2 min of agitation), resuspended in culture media, and seeded in separate wells of a 6-well plate for a 24-h incubation period at 37 degrees C. After 24 h, the thawed samples were analyzed by bright-field microscopy and flow cytometry. The results suggest that the absence of DMSO (and the presence of MC) significantly increases the fraction of apoptotic and/or necrotic ASCs. However, the percentage of viable cells obtained with 2% DMSO and DMEM was comparable with that obtained in freezing media with 10% DMSO and 80% serum (HS or FCS), that is, approximately 84% +/- 5% and approximately 84% +/- 8%, respectively. Adipogenic and osteogenic differentiation behavior of the frozen thawed cells was also assessed using histochemical staining. Our results suggest that post-thaw ASC viability, adipogenic and osteogenic differentiability can be maintained even when they are frozen in the absence of serum but with a minimal concentration of 2% DMSO in DMEM.

Topics: Adipocytes, White; Adult Stem Cells; Apoptosis; Cell Differentiation; Cell Separation; Cell Survival; Cells, Cultured; Cryopreservation; Cryoprotective Agents; Culture Media, Serum-Free; Dimethyl Sulfoxide; Humans; Methylcellulose; Necrosis; Osteocytes

The effects of experimental hypertension on retinal cells were studied. Evaluation was made of IOP levels and degree of cell damage by cytochemical and DNA analysis, and degeneration modes: necrosis and apoptosis.

Topics: Acute Disease; Animals; Apoptosis; DNA; Follow-Up Studies; Intraocular Pressure; Male; Methylcellulose; Necrosis; Ocular Hypertension; Rats; Retina; Retinal Degeneration

A marked increase of serum transaminase activities, histological changes of livers similar to those seen in viral hepatitis in man, and inhibition of hepatic protein synthesis were observed in rats following a single injection of D-galactosamine-HCl. These galactosamine-induced phenomena were prevented by the pretreatment of polyriboinosinic acid-polyribocytidylic acid 24 h before the galactosamine administration.

Topics: Alanine Transaminase; Animals; Aspartate Aminotransferases; Carboxymethylcellulose Sodium; Chemical and Drug Induced Liver Injury; Galactosamine; Liver; Liver Diseases; Male; Methylcellulose; Necrosis; Peptides; Poly I-C; Polylysine; Rats; Rats, Inbred Strains

Topics: Anesthetics, Local; Angiography; Animals; Capillary Permeability; Contrast Media; Dextrans; Diatrizoate; Ear, External; Lidocaine; Mepivacaine; Methods; Methylcellulose; Microcirculation; Microradiography; Necrosis; Rabbits; Regional Blood Flow; Sodium Chloride; Thermography; Time Factors; Transillumination

Topics: Animals; Antibodies; Body Weight; Carbon Isotopes; Complement System Proteins; Guinea Pigs; Leukocytes; Methylcellulose; Necrosis; Norepinephrine; Opsonin Proteins; Phagocytosis; Salmonella typhimurium; Shigella; Sodium Chloride; Staphylococcus; Sympathetic Nervous System

Topics: Adrenal Medulla; Animals; Cricetinae; Cytoplasm; Endoplasmic Reticulum; Female; Golgi Apparatus; Histocytochemistry; Male; Methods; Methylcellulose; Microscopy; Microscopy, Electron; Mitochondria; Necrosis; Ribosomes

Topics: Adrenal Glands; Adrenalectomy; Animals; Benz(a)Anthracenes; Corticosterone; Heparin; Heparin Antagonists; In Vitro Techniques; Liver; Methylcellulose; Methylcholanthrene; Necrosis; Oxidoreductases; Polyvinyls; Povidone; Quaternary Ammonium Compounds; Rats; Sulfonic Acids