methylcellulose and Lymphoma

This study demonstrated the importance of the methods used in determining the lymphoma cell colony stimulating activity of factors derived from lymphoma cells. The in vitro colony formation in a semisolid matrix of the AKR mouse lymphoma cell line, SL 12, and three cloned derivatives, SL 12.1, SL 12.3, and SL 12.4, was studied. We show that the use of soft agar or methylcellulose as a semisolid matrix results in colony formation by the lymphoma cells only in the presence of serum. The addition of conditioned medium (CM) from lymphoma cells growing in serum-free medium does not stimulate colony growth. However, when purified agarose is used, colonies grow in a dose-dependent manner in the absence of serum and in the presence of CM. These results indicate that the type of semisolid matrix used can influence results in studies of this nature. Purified agarose provides the best environment when colony formation by lymphoma cells is used to measure the presence of growth factors in test-conditioned media.

Topics: Agar; Animals; Cell Division; Cell Line; Clone Cells; Colony-Forming Units Assay; Culture Techniques; Lymphoma; Methylcellulose; Mice

The relationship between the tumorigenic potential and the cloning efficiency of T lymphoma BW5147 in semi-solid media has been studied. Two stable variants exhibiting a 30-fold decrease of their cloning efficiencies in agarose an methylcellulose media were independently isolated by negative selection with FuDr. These variants show no alteration of their growth properties in liquid medium and are still able to proliferate in liquid suspension over a bottom layer of agarose. This new phenotype is not correlated with any decreased tumorigenicity in syngeneic AKR/J mice.

Topics: Animals; Cell Division; Cell Line; Cell Separation; Culture Media; Lymphoma; Methylcellulose; Mice; Neoplasm Transplantation; Phenotype; Sepharose

Topics: Blood Pressure Determination; Blood Proteins; Lymphoma; Lymphoma, Non-Hodgkin; Methylcellulose; Neoplasms; Neoplasms, Experimental

Topics: Lymphoma; Lymphoma, Non-Hodgkin; Methylcellulose; Neoplasm Regression, Spontaneous; Neoplasms; Rats; Research

Topics: Animals; Lymphoma; Lymphoma, Non-Hodgkin; Methylcellulose; Neoplasms, Experimental; Rats