methylcellulose and Lymphoma--Non-Hodgkin

The aim of this study was to assess the diagnostic potential of multidetector computed tomography (MDCT) in the evaluation of small-bowel neoplasms.. We studied 120 patients with suspected small-bowel disease by 16-slice MDCT after oral administration of a polyethylene glycol solution (n=56) or methylcellulose via a nasojejunal tube (n=64). Unenhanced and contrast-enhanced CT was performed. Contrast-enhanced CT images were acquired 40 s after IV injection of 130 ml of iodinated contrast agent at a rate of 3 ml/s. Multiplanar reconstructions were performed at the end of the examinations.. Fifteen patients were found to be affected by small-bowel neoplasm (six had non-Hodgkin's lymphoma, three had carcinoid tumour, two had Peutz-Jeghers syndrome, two had adenocarcinoma, two had melanoma metastases, one had lipoma). In the remaining patients, 58 cases of Crohn's disease and seven miscellaneous diseases were detected. All findings were confirmed by barium studies, surgery or endoscopy.. MDCT performed after bowel-loop distension with low-density contrast material and IV administration of iodinated contrast agent is a reliable method for diagnosing and staging small-bowel neoplasms.

Topics: Adenocarcinoma; Administration, Oral; Barium Sulfate; Biopsy; Carcinoid Tumor; Chi-Square Distribution; Contrast Media; Enema; Female; Humans; Ileal Neoplasms; Ileum; Image Processing, Computer-Assisted; Injections, Intravenous; Jejunal Neoplasms; Jejunum; Lipoma; Lymphoma, Non-Hodgkin; Male; Methylcellulose; Peutz-Jeghers Syndrome; Polyethylene Glycols; Radiographic Image Enhancement; Sensitivity and Specificity; Tomography, X-Ray Computed

A major goal of experimental and clinical hematology is the identification of mechanisms and conditions supporting the expansion of transplantable hematopoietic stem cells. We assessed the expansion potential of CD34+CD71-CD45- cells derived from granulocyte colony-stimulating factor (G-CSF)-mobilized peripheral blood under recently defined serum-free culture conditions. The CD34+CD71-CD45- cells in mobilized peripheral blood were found to contain the majority (92%+/-5.6) of primitive long-term culture initiating cells (LTCIC) and 53.5%+/-16.7 of the more committed colony-forming cells (CFC). Furthermore, this population represents 23.3%+/-4.1 of the total CD34+ cells and allows reduction of the cell density important for maintenance/expansion of primitive progenitor cells. CD34+ CD71- CD45- cells were cultured in defined serum-free media supplemented with 300 ng each of Flt-3 ligand and stem cell factor (SCF), 60 ng of interleukin (IL)-3, and 20 ng each of IL-6 and G-CSF. Mononuclear cells (MNC) and CFC were expanded 50-fold and 200-fold, respectively; primitive progenitor cells (LTC-IC) were maintained at input values after a total of 10 days of expansion. The addition of IL-15 to our cytokine cocktail expanded LTC-IC 2- to 3-fold and CFC to >500-fold. The data presented should allow clinical manipulation (purging) and expansion procedures with mobilized PBPC harvests without the loss of primitive progenitor cells and could be made applicable for large-scale clinical expansion.

Topics: ADP-ribosyl Cyclase; ADP-ribosyl Cyclase 1; Antigens, CD; Antigens, CD34; Antigens, Differentiation, B-Lymphocyte; Breast Neoplasms; Cells, Cultured; Culture Media, Serum-Free; Erythropoietin; Granulocyte Colony-Stimulating Factor; Granulocyte-Macrophage Colony-Stimulating Factor; Hematopoietic Stem Cells; Humans; Interleukin-15; Interleukin-3; Interleukin-6; Leukocyte Common Antigens; Lymphoma, Non-Hodgkin; Membrane Glycoproteins; Membrane Proteins; Methylcellulose; Receptors, Transferrin; Stem Cells

Aim of the study was to compare the visualisation of small bowel tumours particularly lymphoma of the small bowel by enteroclysma, computed tomography (CT) and computed tomography following enteroclysma.. We examined 97 examinations in 63 patients. Non Hodgkin's Lymphoma was the primary malignancy in 44 patients, metastasis of different malignancies in 8 patients, primary malignancies of the small intestine in 5 patients, mesenteric tumours in 4 patients and postoperative stricture in another 2 patients. CT following enteroclysma (CT Sellink) was performed as helical CT in 55 patients and as incremental CT in 42 patients. Examinations were evaluated by two radiologists. Evaluation criteria were small bowel distension, perceptibility of details and topographic correlation.. Manifestations of lymphoma were found in 32 patients, infiltration of bowel wall in 12 patients. In three patients metastases of melanoma were found. In three patients the suspicion for small bowel tumours was not verified in CT Sellink. The perceptibility of details was evaluated as ameliorated in 45.5% of examinations over all.. CT Sellink offers remarkable advantages in the diagnosis of small bowel tumours compared with enteroclysma and "conventional" computed tomography under intra-venous and oral contrast media. CT Sellink was feasible over a time of 7 years now in clinical praxis. This examination represents an optimized standard in small intestine examination.

Topics: Adult; Aged; Contrast Media; Female; Humans; Image Processing, Computer-Assisted; Intestinal Neoplasms; Intestine, Small; Iohexol; Lymphoma, Non-Hodgkin; Male; Methylcellulose; Middle Aged; Radiographic Image Enhancement; Sensitivity and Specificity; Tomography, X-Ray Computed

It is well known that ABMT in acute myeloid leukemia (AML) often results in delayed hematopoietic engraftment, but the reason behind this has not been resolved. Previous studies have largely dealt with measurements of committed myeloid progenitors as surrogate markers for hematopoiesis.. Measurements of Week 5 cobblestone area forming cells (CAFC) and stromal-cell growth in BM autografts from 14 AML patients were compared with those from 10 NHL patients.. Grafts achieved from the AML patients contained a significantly lower total number of CAFC than those from the NHL patients. The reason for this was a lower total amount of mononuclear cells (MNC) obtained during harvest procedure (mean 0.4 x 10(8)/kg for AML, versus 0.8 x 10(8)/kg for NHL). In contrast, the frequency of CAFC was comparable both between patient groups (mean 1.47, range 0.15-6.33 per 10(4) MNC for AML versus mean 1.47, range 0.53-3.57 per 10(4) MNC for NHL) and compared with that of eight normal donors (mean 1.12, range 0.73-1.73 per 10(4) MNC). An inverse relationship was observed between the total CAFC number in the grafts and the hematopoietic reconstitution of both granulocytes > or = 2.0 x 10(9)/L and thrombocytes > or = 50 x 10(9)/L, in which the level of 9.0 x 10(3) CAFC/kg implied a prompt engraftment for both patient groups. Whereas the stromal cell outgrowth in vitro from 8/10 NHL patients was similar to that of six normal donors, only a few stromal cells appeared in the majority of nine evaluable AML patients.. A decreased total CAFC content, as well as an inferior stromal-cell function, may be critical elements for prolonged hematopoietic reconstitution in AML.

Topics: Adult; Blood Platelets; Bone Marrow Cells; Bone Marrow Transplantation; Cell Division; Humans; Leukemia, Myeloid, Acute; Lymphoma, Non-Hodgkin; Methylcellulose; Middle Aged; Neutrophils; Stromal Cells; Time Factors

The effects of recombinant products of granulocyte colony-stimulating factors (G-CSF), granulocyte/macrophage CSF (GM-CSF), human interleukin-3 (IL-3), and interleukin-1 (IL-1) were studied using purified target cell populations from patients undergoing peripheral blood stem cell transplantation after myeloablative therapy. Cells were subjected to combined purification procedures including negative selection with a panel of monoclonal antibodies (CD2, 3, 5, 10, and 20). The purified cells were enriched for HLA-DR+ (51% to 71%) and My-10+ (CD34; 37% to 54%) and had a plating efficiency of up to 20%. In the liquid-suspension limiting dilution clonal assay (LDA), purified progenitors responded directly to IL-3 by proliferation with single-hit kinetics. The ability of GM-CSF to support progenitor growth was inferior to that of IL-3, and the cells were virtually unresponsive when cultured with G-CSF, supporting the notion that these blood-derived progenitors belong to a primitive population of hematopoietic progenitor cells. The results obtained in simultaneous methycellulose cultures (MC) showed the same trend and provided additional information on the ability of GM-CSF and IL-3 to support erythroid progenitor growth. The combination of IL-3 plus G-CSF, but not IL-3 plus GM-CSF, resulted in a synergistic increase in colony number. IL-1 alpha increased both the size and number of colonies when added to IL-3 or G-CSF. Study of this enriched progenitor cell population in MC and LDA represents an excellent model for the investigation of myeloid and erythroid differentiation and for evaluating the influence of various cytokines on human hematopoiesis.

Topics: Antigens, CD; Antigens, CD34; Antigens, Differentiation; Cell Division; Cell Separation; Cells, Cultured; Child; Colony-Stimulating Factors; Granulocyte Colony-Stimulating Factor; Granulocyte-Macrophage Colony-Stimulating Factor; Growth Substances; Hematopoietic Stem Cells; HLA-DR Antigens; Humans; Interleukin-1; Interleukin-3; Kinetics; Leukemia; Lymphoma, Non-Hodgkin; Methylcellulose; Neuroblastoma; Recombinant Proteins

26 children with acute lymphoblastic leukemia (ALL) and 7 additional patients with lymphosarcoma in leukaemic transformation (LSA) have been studied with respect to the content of myelopoietic stem cell (CFUc) in blood and bone marrow. The methylcellulose culture technique (Iscove et al 1974) was employed in the absence of an exogenous source of colony stimulating factor (CSF). During active disease, CFUc colony formation was absent from patients with ALL, but was present in 2 patients with LSA. 2 therapeutic regimens were employed. Colony formation from bone marrow CFUc was highly variable during remission maintained by either regimen, with no clear relation to clinical stage, number of monocytes or circulating neutrophils. Patients with LSA consistently had high numbers of bone marrow CFUc. CFUc were low or absent from the blood. In conclusion, CFUc are absent from the bone marrow in active ALL, but may be present in active LSA. For the purpose of monitoring children with ALL during therapy, determination of blood or bone marrow CFUc was not found in this study to be helpful.

Topics: Antineoplastic Agents; Bone Marrow Cells; Cells, Cultured; Child; Colony-Stimulating Factors; Hematopoietic Stem Cells; Humans; Leukemia, Lymphoid; Leukocyte Count; Lymphoma, Non-Hodgkin; Methylcellulose

Topics: Adenocarcinoma; Amino Acids; Animals; Cattle; Chickens; DNA, Neoplasm; Fibrosarcoma; Lymph Nodes; Lymphoma, Non-Hodgkin; Mammary Neoplasms, Experimental; Methylcellulose; Neoplasm Transplantation; Neoplasms, Experimental; Rats; Sarcoma, Avian; Sarcoma, Experimental; Xanthines

Topics: Acrylates; Animals; Cellulose; Centrifugation, Density Gradient; Chemical Phenomena; Chemical Precipitation; Chemistry; Chromatography, Gel; Chromatography, Ion Exchange; Chromatography, Paper; Dextrans; Drug Stability; Electrophoresis; Hydrogen-Ion Concentration; Lymphoma, Non-Hodgkin; Methods; Methylcellulose; Mice; Molecular Weight; Neoplasms, Experimental; Polynucleotides; Ribonucleases; Spleen; Swine; Uracil Nucleotides

Topics: Agar; Animals; Animals, Laboratory; Arthritis, Infectious; Cricetinae; Culture Media; Culture Techniques; Disease Models, Animal; Freund's Adjuvant; Guinea Pigs; Injections; Injections, Intraperitoneal; Lymphoma, Non-Hodgkin; Methylcellulose; Mice; Mucins; Mycoplasma; Mycoplasma Infections; Rabbits; Rats; Sarcoma, Experimental; Sepsis; Silicon Dioxide; Talc; Virulence

Topics: Adrenal Cortex Hormones; Animals; Catalysis; Cellulose; Chemical Precipitation; Chromatography, Ion Exchange; Chromatography, Paper; Chromatography, Thin Layer; Hydrogen-Ion Concentration; Lymphoma, Non-Hodgkin; Methylcellulose; Mice; Neoplasms, Experimental; Nucleotides; Phosphoric Monoester Hydrolases; Protamines; RNA

Topics: Animals; Carcinoma 256, Walker; Female; Lymphoma, Non-Hodgkin; Methylcellulose; Rats

Topics: Animals; Estradiol; Friends; Leukemia; Leukemia Virus, Murine; Leukemia, Experimental; Lymphoma, Large B-Cell, Diffuse; Lymphoma, Non-Hodgkin; Mercaptopurine; Methylcellulose; Mice; Mitomycin; Mitomycins; Neoplasm Transplantation; Neoplasms; Pharmacology; Research; Sarcoma; Triethylenemelamine; Urethane

Topics: Blood Pressure Determination; Blood Proteins; Lymphoma; Lymphoma, Non-Hodgkin; Methylcellulose; Neoplasms; Neoplasms, Experimental

Topics: Lymphoma; Lymphoma, Non-Hodgkin; Methylcellulose; Neoplasm Regression, Spontaneous; Neoplasms; Rats; Research

Topics: Animals; Lymphoma; Lymphoma, Non-Hodgkin; Methylcellulose; Neoplasms, Experimental; Rats