methylcellulose and Leukemia--Lymphoid

Topics: Acute Disease; Agar; Animals; B-Lymphocytes; Cells, Cultured; Chronic Disease; Colony-Forming Units Assay; Humans; Leukemia, Lymphoid; Methylcellulose; Multiple Myeloma

A preliminary study of the culture of leukemic progenitor cells (CFU-L) from acute non-T lymphoblastic leukemia was undertaken for 25 patients (19 were considered in complete remission and 6 in the acute phase of the disease). Two culture systems were tested; a double layer agar-liquid phase and a single layer of methylcellulose. The major problem was the characterization of the colonies: immunological labelling coupled with cytofluorometry, as well as cytomorphology, cytogenetic and more recently molecular biology may allow the characterization of the CFU-L. The culture of CFU-L appears to be an efficient method for detecting residual leukemic cells which can be used to evaluate the quality of both the remission obtained and that of autologous bone marrow after purging.

Topics: Agar; Colony-Forming Units Assay; Culture Media; Growth Substances; Humans; Leukemia, Lymphoid; Leukocytes; Lymphocyte Depletion; Methylcellulose; T-Lymphocytes; Tumor Stem Cell Assay

In the present study we utilized a semisolid culture system with feeder cells and enriched media to evaluate the growth of acute leukemia associated with the 4;11 chromosomal translocation. We compared growth of t(4;11) leukemia to typical acute nonlymphocytic leukemia (ANL) and acute lymphocytic leukemia (ALL). The two cases of t(4;11) leukemia tested exhibited the highest cloning efficiency of cells tested. The growth characteristics of t(4;11) leukemia were more similar to ANL than ALL.

Topics: Acute Disease; Adolescent; Adult; Bone Marrow; Cells, Cultured; Child; Child, Preschool; Chromosomes, Human, 4-5; Chromosomes, Human, 6-12 and X; Colony-Forming Units Assay; Female; Humans; Infant; Leukemia; Leukemia, Lymphoid; Leukemia, Myeloid, Acute; Male; Methylcellulose; Monocytes; Phenotype; Recurrence; Staining and Labeling; Translocation, Genetic

A Phase II study of poly(I,C)-LC was performed in 28 children and adolescents with acute lymphoblastic leukemia (ALL), 10 with acute nonlymphoblastic leukemia (ANLL), and 13 with metastatic neuroblastoma. All were refractory to standard chemotherapeutic agents and 25 to an investigational drug. Initial doses of 12 mg/m2 and 9 mg/m2 were intolerable. However, 9 mg/m2 was tolerable in the majority of patients when the drug was started at 3 mg/m2 and increased by 3 mg/m2 increments. Fifteen children with ALL, three with ANLL, and two with neuroblastoma received the drug daily. Seven patients with ANLL and seven children with neuroblastoma received the drug biweekly. Twenty-eight patients received an adequate trial, which was defined as a minimum of 5 weeks at the maximal tolerated dose, unless there was progressive disease at the maximal tolerated dose. Side effects of the drug were striking, and included fever, hypotension, myalgia, bone pain, arthralgia, arthritis, abdominal pain, liver toxicity, thrombocytopenia, and neurotoxicity. No complete remissions occurred in spite of interferon levels above 100 U in nearly 50% of patients.

Topics: Acute Disease; Carboxymethylcellulose Sodium; Child; Drug Evaluation; Female; Humans; Interferon Inducers; Interferons; Leukemia; Leukemia, Lymphoid; Male; Methylcellulose; Neuroblastoma; Poly I-C; Polylysine

The presence of the common acute lymphoblastic leukemia antigen (CALLA) on leukemic cells from the great majority of patients with non-T cell acute lymphoblastic leukemia and chronic myelogenous leukemia in blast crisis suggests that CALLA could be differentiation antigen expressed by normal lymphoid and myeloid stem cells. Treatment with a murine monoclonal anti-CALLA antibody and complement lysed CALLA-positive leukemic cells quantitatively, whereas similar treatment of nucleated cells from peripheral blood and bone marrow failed to affect the expression, in semisolid culture, of CFU-G/E, BFU-E, CFU-E, or CFU-C. These data suggest that CALLA is not a normal differentiation antigen of the myeloid bipotent cell or its committed progenitors.

Topics: Acute Disease; Antigens, Neoplasm; Blood Coagulation; Bone Marrow Cells; Cell Differentiation; Cells, Cultured; Colony-Forming Units Assay; Cytotoxicity, Immunologic; Erythrocytes; Granulocytes; Hematopoiesis; Humans; Leukemia, Lymphoid; Methylcellulose

26 children with acute lymphoblastic leukemia (ALL) and 7 additional patients with lymphosarcoma in leukaemic transformation (LSA) have been studied with respect to the content of myelopoietic stem cell (CFUc) in blood and bone marrow. The methylcellulose culture technique (Iscove et al 1974) was employed in the absence of an exogenous source of colony stimulating factor (CSF). During active disease, CFUc colony formation was absent from patients with ALL, but was present in 2 patients with LSA. 2 therapeutic regimens were employed. Colony formation from bone marrow CFUc was highly variable during remission maintained by either regimen, with no clear relation to clinical stage, number of monocytes or circulating neutrophils. Patients with LSA consistently had high numbers of bone marrow CFUc. CFUc were low or absent from the blood. In conclusion, CFUc are absent from the bone marrow in active ALL, but may be present in active LSA. For the purpose of monitoring children with ALL during therapy, determination of blood or bone marrow CFUc was not found in this study to be helpful.

Topics: Antineoplastic Agents; Bone Marrow Cells; Cells, Cultured; Child; Colony-Stimulating Factors; Hematopoietic Stem Cells; Humans; Leukemia, Lymphoid; Leukocyte Count; Lymphoma, Non-Hodgkin; Methylcellulose

Topics: Anemia, Aplastic; Bone Marrow Cells; Cell Count; Cell Division; Clone Cells; Humans; In Vitro Techniques; Kinetics; Leukemia, Lymphoid; Leukemia, Myeloid, Acute; Leukocytes; Methylcellulose

Topics: Bone Marrow; Bone Marrow Cells; Cell Line; Culture Media; Culture Techniques; Embryo, Mammalian; Humans; Kidney; Leukemia, Lymphoid; Leukemia, Myeloid; Leukemia, Myeloid, Acute; Leukocytes; Methylcellulose; Monocytes