methylcellulose and Leukemia--Hairy-Cell

methylcellulose has been researched along with Leukemia--Hairy-Cell* in 2 studies

Other Studies

2 other study(ies) available for methylcellulose and Leukemia--Hairy-Cell

Article	Year
Colony growth of normal and neoplastic cells in various concentrations of methylcellulose. Experimental hematology, 1988, Volume: 16, Issue:11 To assess the semisolid character of methylcellulose (MC) and its ability to prevent cell migration and aggregation in clonogenic assays, we studied the influence of various concentrations of MC (0.7%-1.26%) on colony growth of neoplastic cell lines, normal bone marrow cells, and hairy cell leukemia (HCL). All cell lines (K562, HL-60, JOK-1, Daudi, and BB3, an IgM-kappa B-cell line) showed a prominent decrease in colony numbers and remarkable changes in colony morphology at rising MC concentrations, whereas no such influence could be demonstrated for HCL, mixed lineage colony-forming units (CFU-GEMM), granulocyte-macrophage CFU (CFU-GM), erythroid burst-forming units (BFU-E), and erythroid CFU (CFU-E). Despite a decrease in colony numbers at high MC concentrations, some cell lines showed a sustained proliferation as measured by growth index calculations and bromodeoxyuridine (BrdUrd) incorporation. This indicates that at certain MC concentrations colony formation is not always a reflection of proliferation. BrdUrd incorporation yielded an extremely low proliferation capacity for HCL. It is likely that HCL cells, which strongly aggregate, formed pseudo-colonies in spite of high MC concentrations. Topics: Bone Marrow Cells; Cell Count; Cell Line; Colony-Forming Units Assay; Culture Media; Humans; Leukemia, Hairy Cell; Methylcellulose	1988
Human clonogenic cells in vitro. I. Improved preparatory techniques for the collection and concentration of leukemic colonies from methylcellulose cultures for morphologic, cytokinetic, and histochemical evaluation. Stem cells, 1982, Volume: 2, Issue:3 We have developed simple methods for the rapid isolation and concentration of human leukemic colonies for cytologic evaluation. Colonies, grown in a medium containing methylcellulose, are gently washed from their culture dishes after reducing the viscosity of the colony layer. Colonies are washed and concentrated intact by mild centrifugation (10-20 g/5 min). Once isolated, colonies may enter any number of procedures for morphologic and cytokinetic analyses including exposure to radiolabeled compounds, cytocentrifugation, reaction with antibodies specific for certain cell surface markers, and fixation for routine light and electron microscopy. In addition, suspended or smeared colonies may be exposed to various substrates for the demonstration of endogenous enzymatic markers. Examples of possible preparations are presented. Topics: Cell Separation; Cell Transformation, Neoplastic; Cells, Cultured; Clone Cells; Hematopoiesis; Humans; Interphase; Leukemia, Hairy Cell; Leukemia, Myeloid, Acute; Methylcellulose	1982

Article

Year

Colony growth of normal and neoplastic cells in various concentrations of methylcellulose.

Experimental hematology, 1988, Volume: 16, Issue:11

To assess the semisolid character of methylcellulose (MC) and its ability to prevent cell migration and aggregation in clonogenic assays, we studied the influence of various concentrations of MC (0.7%-1.26%) on colony growth of neoplastic cell lines, normal bone marrow cells, and hairy cell leukemia (HCL). All cell lines (K562, HL-60, JOK-1, Daudi, and BB3, an IgM-kappa B-cell line) showed a prominent decrease in colony numbers and remarkable changes in colony morphology at rising MC concentrations, whereas no such influence could be demonstrated for HCL, mixed lineage colony-forming units (CFU-GEMM), granulocyte-macrophage CFU (CFU-GM), erythroid burst-forming units (BFU-E), and erythroid CFU (CFU-E). Despite a decrease in colony numbers at high MC concentrations, some cell lines showed a sustained proliferation as measured by growth index calculations and bromodeoxyuridine (BrdUrd) incorporation. This indicates that at certain MC concentrations colony formation is not always a reflection of proliferation. BrdUrd incorporation yielded an extremely low proliferation capacity for HCL. It is likely that HCL cells, which strongly aggregate, formed pseudo-colonies in spite of high MC concentrations.

Topics: Bone Marrow Cells; Cell Count; Cell Line; Colony-Forming Units Assay; Culture Media; Humans; Leukemia, Hairy Cell; Methylcellulose

1988

Human clonogenic cells in vitro. I. Improved preparatory techniques for the collection and concentration of leukemic colonies from methylcellulose cultures for morphologic, cytokinetic, and histochemical evaluation.

Stem cells, 1982, Volume: 2, Issue:3

We have developed simple methods for the rapid isolation and concentration of human leukemic colonies for cytologic evaluation. Colonies, grown in a medium containing methylcellulose, are gently washed from their culture dishes after reducing the viscosity of the colony layer. Colonies are washed and concentrated intact by mild centrifugation (10-20 g/5 min). Once isolated, colonies may enter any number of procedures for morphologic and cytokinetic analyses including exposure to radiolabeled compounds, cytocentrifugation, reaction with antibodies specific for certain cell surface markers, and fixation for routine light and electron microscopy. In addition, suspended or smeared colonies may be exposed to various substrates for the demonstration of endogenous enzymatic markers. Examples of possible preparations are presented.

Topics: Cell Separation; Cell Transformation, Neoplastic; Cells, Cultured; Clone Cells; Hematopoiesis; Humans; Interphase; Leukemia, Hairy Cell; Leukemia, Myeloid, Acute; Methylcellulose

1982