methylcellulose and Hemolysis

Microencapsulation is a potential biotechnological tool, which can overcome antimicrobial peptides (AMP) instabilities and reduce toxic side effects. Thus, this study evaluates the antibacterial activities of the Ctx(Ile

Topics: Acinetobacter baumannii; Alginates; Drug Compounding; Food Additives; Hemolysis; Methylcellulose; Microbial Sensitivity Tests; Particle Size; Pore Forming Cytotoxic Proteins; Pseudomonas aeruginosa; Salmonella; Staphylococcus aureus

The collaborative endeavor in tissue engineering is to fabricate a bio-mimetic extracellular matrix to assist tissue regeneration. Thus, a novel injectable tissue scaffold was fabricated by exploring nanotailored hyaluronic acid (nHA) and methylcellulose (MC) (nHAMC) along with pristine HA based MC scaffold (HAMC). nHA with particle size ∼22 ± 5.3 nm were obtained and nHAMC displayed a honeycomb-like 3D microporous architecture. Nano-HA bestowed better gel strength, physico-rheological and biological properties than HA. It creditably reduced the high content of salt to reduce the gelation temperature of MC. The properties ameliorated with increased in-corporation of nano-HA. The addition of salt showed more prominent effect on gelation temperature of nHAMC than in HAMC; and salting-out effect was dependent on nHA/HA content. Biocompatible nHAMC assisted adequate cell adherence and proliferation with more extended protrusions with better migration rate than control. Thus, biomodulatory effect of nanotailored glycosaminoglycan could be asserted to design an efficient thermo-responsive scaffold.

Topics: Biocompatible Materials; Cell Line; Cell Movement; Cell Proliferation; Erythrocytes; Fibroblasts; Hemolysis; Humans; Hyaluronic Acid; Hydrogels; Methylcellulose; Rheology; Tissue Scaffolds; Wound Healing

The present study was designed to assess and compare with a range of surfactant-coated, nimesulide-free, and nimesulide-loaded ethylcellulose/methylcellulose (EC/MC) nanoparticles that were prepared by varying drug concentration (ED/MD), polymer concentration (EP/MP), and surfactant concentration (ES/MS). EC/MC nanoparticles prepared by desolvation method produced discrete particles and they were characterized by SEM, AFM, and FTIR studies. The particles mean size diameter (nm) ranged from 244 to 1056 nm and 1065 to 1710 nm for EC and MC nanoparticles, respectively. Studies on drug: polymer ratio showed a linear relationship between drug concentration and percentage of loading in nanoparticles. The encapsulation efficiency decreased with the increase of nimesulide concentration with respect to polymer concentration. Encapsulation efficiency of drug-loaded nanoparticles was varied between 32.8% and 64.9%. The in vitro release of drug-loaded nanoparticles was found to be a first order. This was significantly increased in EC nanoparticles (95.50%) in comparison with MC nanoparticles (95.12%) after 12 h in 24 h long study. Nimesulide release from EC nanoparticles was much slower at slightly alkaline pH 7.4. The in vitro hemolysis tests of nanoparticles were carried out to ascertain the hemocompatibility and shown to be insignificant for EC nanoparticles. In comparison, ES4 from EC formulations with nimesulide was found to be promising with slow and sustained drug release.

Topics: Administration, Oral; Anti-Inflammatory Agents, Non-Steroidal; Cellulose; Drug Carriers; Hemolysis; Humans; Methylcellulose; Microscopy, Atomic Force; Nanoparticles; Particle Size; Spectroscopy, Fourier Transform Infrared; Sulfonamides

Human umbilical cord blood (UCB) has been successfully used as an alternative source of allogeneic hematopoietic stem cells for pediatric transplantation. Clinical banking of UCB requires volume reduction and red cell depletion for cost-effective storage. We have compared processing UCB by Ficoll, Percoll, methylcellulose, gelatin, starch, and red cell lysis. As individual UCB collections vary widely in colony forming cell (CFC) and CD34+ cell content, each UCB (n = 26) was processed by three or more techniques in parallel with Ficoll as the "standard" method. Gelatin gave a consistently high recovery of CFC (92%) and CD34+ cells (86%). Between 0.10-2.50% of the leukocytes in gelatin-treated UCB were CD34+ with an intra-assay variation of 2.1%. Combining data from individual experiments, the correlation between CD34+ and CFC content was excellent (r = 0.77). Lysis rated second in terms of CD34+ and CFC recoveries but is not as practical because of the large volumes involved. Ficoll and Percoll came third but are more expensive and more involved techniques. Starch sedimentation proved to be slow, while methylcellulose processing lost over 60% of CFC and CD34+ cells. After gelatin processing, we calculated 70-mL donations of UCB would contain a mean +/- SD of 9 +/- 2 x 10(8) nucleated cells, 32 +/- 18 x 10(5) CD34+ cells, and 20 +/- 12 x 10(5) CFC with greater than 95% red cell depletion. Recent published computer studies suggest that as few as 2 x 10(5) CD34+ cells may be needed for sustained engraftment of allogeneic marrow in adult transplant recipients. We conclude that average 70-mL UCB donations contain sufficient marrow repopulating cells for adult recipients.

Topics: Antigens, CD34; Centrifugation, Density Gradient; Erythrocyte Count; Erythrocytes; Female; Fetal Blood; Ficoll; Gelatin; Hemolysis; Humans; Infant, Newborn; Labor Stage, Third; Methylcellulose; Povidone; Pregnancy; Silicon Dioxide; Specimen Handling; Starch; Stem Cells

Extensive general pharmacological studies of hydroxypropylmethylcellulose acetate succinate (HPMCAS) were carried out in mice, rats, guinea pigs, rabbits, dogs and frogs. HPMCAS appeared to have no significant effect on the central nervous system, autonomic nervous system and cardiovascular system. Various biological analyses of the blood (including hemolysis and coagulation properties) and urine were unaffected, and the compound showed no significant local anesthetic or vascular permeability. At higher doses of HPMCAS, an increase in secretion of saliva in guinea pigs, a decrease in gastric juice secretion in rats and an increase in rectal temperature in rats were observed, but these effects did not show clear dose-dependence.

Topics: Animals; Autonomic Nervous System; Blood Chemical Analysis; Brain; Capillary Permeability; Digestive System; Dogs; Electrolytes; Guinea Pigs; Hemodynamics; Hemolysis; In Vitro Techniques; Male; Methylcellulose; Mice; Rabbits; Rats

Topics: Amino Acids; Chemical Precipitation; Chromatography, DEAE-Cellulose; Chromatography, Gel; Cysteine; Dextrans; Electrophoresis, Disc; Hemolysin Proteins; Hemolysis; Immunodiffusion; Listeria monocytogenes; Methods; Methylcellulose; Spectrophotometry, Ultraviolet; Temperature; Ultracentrifugation

Topics: Animals; Blood Platelets; Chromium Radioisotopes; Erythrocytes; Hemoglobins; Hemolysis; Hypersplenism; Leukocytes; Male; Methylcellulose; Rats; Reticulocytes

Topics: Amino Acid Sequence; Amino Acids; Animals; Blood Protein Electrophoresis; Caniformia; Chromatography; Chromatography, Gel; Electrophoresis, Starch Gel; Erythrocytes; Hemoglobins; Hemolysis; Hot Temperature; Methylcellulose; Molecular Weight; Protein Denaturation

Topics: Anesthetics, Local; Animals; Blood Flow Velocity; Capillary Fragility; Capillary Permeability; Cells; Cheek; Contrast Media; Cricetinae; Dextrans; Gels; Hemolysis; Humans; Ischemia; Lidocaine; Male; Mepivacaine; Methods; Methylcellulose; Microcirculation; Radiography; Regional Blood Flow; Solutions; Staining and Labeling; Time Factors; Urethra

Topics: Animals; Anticoagulants; Asbestos; Cell Membrane; Drug Antagonism; Edetic Acid; Escherichia coli; Glycosaminoglycans; Hemolysis; Horses; Humans; In Vitro Techniques; Methylcellulose; Minerals; Polystyrenes; Polyvinyls; Pyrans; Sheep; Silicon Dioxide; Staphylococcus

Topics: Animals; Asbestos; Cell Membrane; Edetic Acid; Erythrocytes; Escherichia coli; Glycosaminoglycans; Hemolysis; Horses; Humans; Hydroxides; Magnesium; Methylcellulose; Polysaccharides; Polystyrenes; Povidone; Sheep; Silicon Dioxide; Staphylococcus

Topics: Blood Cells; Cell Membrane Permeability; Dextrans; Erythrocytes; Hemolysis; Humans; Hypotonic Solutions; In Vitro Techniques; Isotonic Solutions; Methylcellulose; Osmotic Fragility; Rheology; Sodium Chloride

Topics: Chromatography, Ion Exchange; Hemoglobins; Hemolysis; Heterozygote; Humans; Methylcellulose; Molecular Biology; Peptides; Sodium; Thalassemia

Topics: Animals; Antibodies; Binding Sites; Chromatography, Ion Exchange; Complement System Proteins; Erythrocytes; Guinea Pigs; Hemolysis; Immune Sera; In Vitro Techniques; Kinetics; Methylcellulose; Sheep; Suramin

Topics: Amino Acids; Animals; Carbon Isotopes; Centrifugation, Density Gradient; Chromatography, Gel; Chromatography, Ion Exchange; Globins; Hemoglobins; Hemolysis; Iron Isotopes; Kinetics; Methylcellulose; Peptide Biosynthesis; Rabbits; Reticulocytes; Ribosomes; RNA, Transfer; Spectrophotometry

Topics: Animals; Antibody Formation; Culture Media; Hemolysis; In Vitro Techniques; Lymphoid Tissue; Methylcellulose; Sheep

Topics: Animals; Hemolysis; Methylcellulose; Rats

Topics: Blood Proteins; Cell Death; Hemolysis; Humans; Lymphoid Tissue; Methylcellulose; Neoplasms; Peptide Nucleic Acids