methylcellulose and Endophthalmitis

In a controlled prospective trial the peroperative use of sodium hyaluronate and methylcellulose (2%) was compared. Two hundred and fifteen patients received sodium hyaluronate and 189 methylcellulose during extracapsular cataract extraction. We found no significant difference in postoperative anterior chamber inflammation, intraocular pressure, frequency of fibrin in the anterior chamber, nor need for additional medication during the first 5 postoperative days.

Topics: Aged; Anterior Chamber; Cataract Extraction; Endophthalmitis; Female; Fibrin; Humans; Hyaluronic Acid; Intraocular Pressure; Male; Methylcellulose; Prospective Studies

To measure the endotoxin concentration (EC) of 25 commercially available, hyaluronic acid- and hydroxypropylmethylcellulose-based (HPMC) ophthalmic viscosurgical devices (OVDs).. The in vitro Limulus amebocyte lysate (LAL) assay, which indicates the presence of endotoxins originating from gram-negative bacteria, was used to determine the EC. The procedure was performed according to the European Pharmacopoeia/USP. EC including duplicate determinations, negative controls, dilution series with control standard endotoxin, dilution series with sample extract and positive sample control.. 16 OVDs (Amvisc, Amvisc Plus, Biolon, Coatel, Healon, Healon GV, Healon, HPMC Ophtal L, Microvisc, Microvisc Plus, Ocucoat, Provisc, Rayvisc, Viscoat, Visco Shield 2%, Visko 1.4%) had an EC under 1.2 endotoxin units/mL, five (Adatocel, HPMC Ophtal H, LA Gel, Viscorneal, Viscorneal Plus) had an EC > or = 1.2 and < or = 24 EU/ml, and four (Biocorneal, Dispasan also named Ophthalin, Dispasan Plus, Visko 1%) had an EC of > 24 EU/ml.. To avoid viscoelastic-related inflammatory or immunological reactions, the use of pure OVDs is recommended, especially for surgical procedures with an inherent possibility of leaving viscoelastic remnants in the eye (e.g., cataract surgery, visco-canalostomy or penetrating keratoplasty).

Topics: Endophthalmitis; Endotoxins; Gram-Negative Bacteria; Humans; Hyaluronic Acid; Lactose; Limulus Test; Methylcellulose; Ophthalmologic Surgical Procedures; Oxazines; Reproducibility of Results; Sensitivity and Specificity

Topics: Anti-Bacterial Agents; Bacterial Infections; Endophthalmitis; Humans; Methylcellulose; Pharmaceutical Vehicles

We evaluated the safety and efficacy of 2% methycellulose as an adjunct for cataract extraction with implantation in cat and monkey models. When used intraoperatively, methylcellulose reduced the iridovitreal bulge during surgery. No significant increase in clinical inflammation occurred nor was there statistically significant intraocular pressure elevation at 24 hours, 7 days, or 90 days. In the cat model, the central corneal thickness increased at day seven in both control and methylcellulose eyes; this thickness persisted to 90 days. The endothelial cell loss decreased significantly at day 90 in methylcellulose eyes. In the monkey model, no statistically significant increase in corneal thickness occurred in control or methylcellulose eyes at day seven. The endothelial cell loss was greater than in the cat model in both control and methylcellulose eyes; there was no statistically significant difference between the two. Two percent methylcellulose was safe in both the cat and monkey models. It facilitated surgery in both models and reduced the endothelial cell loss in the cat eye.

Topics: Animals; Cataract Extraction; Cats; Cell Count; Cornea; Endophthalmitis; Endothelium; Haplorhini; Intraocular Pressure; Lenses, Intraocular; Methylcellulose; Postoperative Complications