methylcellulose and Colonic-Neoplasms

Topics: Aged; Bile Acids and Salts; Carcinoma; Cholelithiasis; Colonic Neoplasms; Constipation; Diet; Dietary Carbohydrates; Diverticulitis; Diverticulum; Feces; Gastrointestinal Diseases; Gastrointestinal Motility; Humans; Methylcellulose; Middle Aged; Polysaccharides

Oxycellulose, a hemostatic agent used in operation showed antitumor effect in vitro on a murine hepatic cell carcinoma (MH 134), a murine fibrosarcoma (Meth A) and a murine colon carcinoma (Colon 26). The effect was also confirmed in vivo by the survival of mice inoculated with Meth A or MH 134. Eighty milligrams per mouse of this agent, however, showed a toxicity rather than an antitumor effect. The antitumor effect of oxycellulose on Meth A did not compare with that of etoposide or mitomycin C in vivo. The antitumor effect on MH 134 was equal to that of etoposide but not mitomycin C. Oxycellulose inhibited tritium thymidine uptake into Colon 26 cells to the same extent as 5-fluorouracil and mitomycin C and it caused 51Cr-labelled Colon 26 cells but not from 5-fluorouracil or mitomycin C. Oxycellulose decreased a larger number of viable tumor cells than 5-fluorouracil or mitomycin C when the tumor cells were incubated for 24 hours at 37 degrees C. DNA histogram with MH 134 cells showed oxycellulose decreased a ratio of tumor cells in S-phase. These results suggest that the antitumor effect of oxycellulose is cytocidal and phase-specific.

Topics: Animals; Antineoplastic Agents; Cell Division; Colonic Neoplasms; DNA, Neoplasm; Erythrocytes; Etoposide; Female; Fibrosarcoma; Hemostatics; Hypromellose Derivatives; Injections, Intraperitoneal; Liver Neoplasms, Experimental; Methylcellulose; Mice; Mice, Inbred BALB C; Mice, Inbred C3H; Mitomycin; Tumor Cells, Cultured

To determine the accuracy of hydrosonography (HUS) und hydro-CT (HCT) for staging colon carcinoma.. 74 patients in whom colorectal carcinoma was suspected were examined. At HUS the colonic wall ist distended by a methylcellulose/water suspension and the carcinoma is enlarged to perform staging of the tumour. HCT is a spiral-CT optimised for parenchymal and vessel contrast. Before the scan is started, up to two litres of fluid are given rectally and spasmolytics are administered to reduce peristalsis. Colorectal carcinomas were classified according to the TNM system and histopathologic correlation was achieved.. Out of 43 (HUS) and 39 (HCT) colonic lesions 33 (77%) and 36 (92%), respectively, were diagnosed. T-stage accuracy was 88% (HUS) and 66% (HCT), N-stage accuracy 33% and 46% and M-stage accuracy 88% and 85%, respectively.. The T-stage of sonographically visible tumours of the colon is determined precisely by HUS. In contrast to predicting lymph node involvement distant metastases are reliably detected by both methods. If performed together, HUS and HCT achieve high diagnostic accuracy for staging carcinoma of the colon.

Topics: Adult; Aged; Aged, 80 and over; Cathartics; Colon; Colon, Sigmoid; Colonic Neoplasms; Contrast Media; Diagnosis, Differential; Evaluation Studies as Topic; Female; Humans; Male; Methylcellulose; Middle Aged; Sensitivity and Specificity; Sigmoid Neoplasms; Tomography, X-Ray Computed; Ultrasonography; Water

A serum-free medium has been developed which supports colony formation by cells from several human tumor cell lines, one colon adenocarcinoma (WiDr) and four melanoma (Me43, Me85, MP6, MeIuso). This medium consists of a 1:1 mixture of an enriched Dulbecco's modified Eagle's medium (EMED) and a modified Ham's F-12 nutrient mixture (FMED) supplemented with 0.9% methylcellulose, 1% bovine serum albumin, 80 micrograms/ml human transferrin, 3 micrograms/ml insulin, 2.8 micrograms/ml linoleic acid, 2.6 micrograms/ml cholesterol, 20 microM ethanolamine, and trace elements. Colony formation by WiDr cells is linear with the numbers of cells plated, having a plating efficiency (PE) of 34%, as compared to 26% in serum-containing medium. Two of the melanoma cell lines. MP6 and MeIuso, exhibit linear relationships between colony numbers and cell concentration with PEs of 21% and 70% respectively. Colony formation by the other two melanoma cell lines appears to be nonlinear. This work represents a step toward standardizing culture conditions for human tumor clonogenic cell assays.

Topics: Adenocarcinoma; Cell Aggregation; Cell Division; Cell Line; Colonic Neoplasms; Colony-Forming Units Assay; Culture Media; Humans; Melanoma; Methylcellulose; Serum Albumin, Bovine; Tumor Stem Cell Assay

Topics: Anthraquinones; Cathartics; Colonic Diseases, Functional; Colonic Neoplasms; Diagnosis, Differential; Diarrhea; Humans; Metabolic Diseases; Methylcellulose; Mineral Oil; Phenolphthaleins

Topics: Barium Sulfate; Cecum; Colon; Colonic Diseases; Colonic Neoplasms; Humans; Methods; Methylcellulose; Posture; Radiography