methylcellulose and Carcinoma

Topics: Carcinoma; Contrast Media; Humans; Magnetic Resonance Imaging; Methylcellulose; Radiography; Rectal Neoplasms

Topics: Aged; Bile Acids and Salts; Carcinoma; Cholelithiasis; Colonic Neoplasms; Constipation; Diet; Dietary Carbohydrates; Diverticulitis; Diverticulum; Feces; Gastrointestinal Diseases; Gastrointestinal Motility; Humans; Methylcellulose; Middle Aged; Polysaccharides

The role of oxidized regenerated methylcellulose (ORC) in the lymphocyst formation after systematic lymphadenectomy.. This was a retrospective case-control study. Patients with gynecologic cancer who underwent systematic lymphadenectomy from May 2000 to April 2006 were considered. Retroperitoneal "no closure" method was performed in all patients. Two groups were identified according to ORC use. The lymphocysts were evaluated via ultrasonography/computed tomography/magnetic resonance imaging between the third and sixth months after surgery.. The overall lymphocyst incidence was found to be 75 (29.8%) of 252, and lymphocyst incidence in the ORC and control groups was 45 (30%) of 150 and 30 (29.4%) of 102, respectively. The mean (SD) total number of extracted lymph nodes in the ORC group was 27.5 (10.6), which was significantly higher than that in the control group (22.1 [10.8]; P = 0.001). Duration of drain was significantly longer in the ORC group (P = 0.028). However, when confounding variables were included into the binary logistic regression analysis for the prediction of the duration of drains, only the stage of disease predicted the duration of drains.. Use of ORC does not seem to affect lymphocyst formation. Oxidized regenerated methylcellulose use does not affect the duration of drains, hence ORC does not seem to pose a stimulatory effect on the peritoneum.

Topics: Carcinoma; Case-Control Studies; Cellulose, Oxidized; Female; Genital Neoplasms, Female; Hemostasis, Surgical; Humans; Lymph Node Excision; Lymphocele; Methylcellulose; Peritoneum; Retrospective Studies

Clonal growth of tumor cell lines originating from a variety of solid tumors was studied. The seeding efficiency of these tumors in methylcellulose medium was in the range of 0.036 to 0.177. Stromal cell lines from mouse bone marrow as well as primary stromal cells from human bone marrow stimulated the growth of HCT and oat human carcinoma cells 32-fold and 25-fold, respectively. In contrast, these stromal cells inhibited the in vitro cloning of human and mouse sarcoma cell lines. Both activities of the stromal cells diffused through agar layers and operated across species barriers. Despite the diffusable nature of the factors involved, no biologic activity was observed in concentrated conditioned media prepared in the presence or absence of serum. Human foreskin fibroblasts tested under identical conditions, could neither stimulate nor inhibit the clonal growth of tumors. This preferential growth of tumor cells in the presence of tissue specific stroma may be used as an in vitro model for the study of the role of stromal cells in tumor cell spread.

Topics: Animals; Carcinoma; Cell Line; Colony-Forming Units Assay; Culture Media; Hematopoietic Stem Cells; Humans; Methylcellulose; Mice; Neoplastic Stem Cells; Sarcoma, Experimental; Tumor Stem Cell Assay

Methods and evaluation of the human tumor stem cell assay (HTSCA) are described. Advantages and disadvantages of the test system are elaborated. The in vitro/in vivo correlation in the drug screening of human ovarian carcinomas shows that the prediction of sensitivity to a cytotoxic agent is only possible in 64%. Prediction of drug resistance, however, seems to be possible in 95%. The number of patients that profit from the HTSCA seems to be only less than 10%. Our investigations describe the influence of various hormones and antiestrogens on the colony formation of human ovarian carcinoma cells. Tamoxifen and his major metabolite 4-hydroxy-tamoxifen were the most active agents. Both compounds inhibit the colony survival (70% at pharmacological concentrations) of 60% of the screened ovarian carcinomas. A significant correlation to the quantitative level of estrogen or progesterone receptors could not be proved. Colony formation of ovarian carcinoma cells was compared in the HTSCA as described by Hamburger and Salmon and in a methylcellulose-monolayer system. Our results show that the colony formation corresponds to the results of the original HTSCA: Cloning ovarian carcinoma cells in the methylcellulose-monolayer, however, seems to be technically easier and faster.

Topics: Agar; Antineoplastic Agents; Carcinoma; Cells, Cultured; Colony-Forming Units Assay; Drug Resistance; Estradiol; Female; Hormones; Humans; Medroxyprogesterone; Medroxyprogesterone Acetate; Methylcellulose; Ovarian Neoplasms; Progesterone; Prognosis; Tamoxifen; Tumor Stem Cell Assay

Topics: Agar; Carcinoma; Cell Division; Cell Survival; Cells, Cultured; Clone Cells; Drug Evaluation, Preclinical; Humans; Karyotyping; Methylcellulose; Mitomycins; Urinary Bladder Neoplasms

Topics: Adenoma; Animals; Body Weight; Carcinoma; Carcinoma, Hepatocellular; Diet; Female; Food Additives; Genital Neoplasms, Female; Liver Neoplasms; Lung Neoplasms; Male; Methylcellulose; Neoplasms; Rats; Skin Neoplasms; Urogenital Neoplasms