methylcellulose and Adenocarcinoma

The aim of this study was to assess the diagnostic potential of multidetector computed tomography (MDCT) in the evaluation of small-bowel neoplasms.. We studied 120 patients with suspected small-bowel disease by 16-slice MDCT after oral administration of a polyethylene glycol solution (n=56) or methylcellulose via a nasojejunal tube (n=64). Unenhanced and contrast-enhanced CT was performed. Contrast-enhanced CT images were acquired 40 s after IV injection of 130 ml of iodinated contrast agent at a rate of 3 ml/s. Multiplanar reconstructions were performed at the end of the examinations.. Fifteen patients were found to be affected by small-bowel neoplasm (six had non-Hodgkin's lymphoma, three had carcinoid tumour, two had Peutz-Jeghers syndrome, two had adenocarcinoma, two had melanoma metastases, one had lipoma). In the remaining patients, 58 cases of Crohn's disease and seven miscellaneous diseases were detected. All findings were confirmed by barium studies, surgery or endoscopy.. MDCT performed after bowel-loop distension with low-density contrast material and IV administration of iodinated contrast agent is a reliable method for diagnosing and staging small-bowel neoplasms.

Topics: Adenocarcinoma; Administration, Oral; Barium Sulfate; Biopsy; Carcinoid Tumor; Chi-Square Distribution; Contrast Media; Enema; Female; Humans; Ileal Neoplasms; Ileum; Image Processing, Computer-Assisted; Injections, Intravenous; Jejunal Neoplasms; Jejunum; Lipoma; Lymphoma, Non-Hodgkin; Male; Methylcellulose; Peutz-Jeghers Syndrome; Polyethylene Glycols; Radiographic Image Enhancement; Sensitivity and Specificity; Tomography, X-Ray Computed

A serum-free medium has been developed which supports colony formation by cells from several human tumor cell lines, one colon adenocarcinoma (WiDr) and four melanoma (Me43, Me85, MP6, MeIuso). This medium consists of a 1:1 mixture of an enriched Dulbecco's modified Eagle's medium (EMED) and a modified Ham's F-12 nutrient mixture (FMED) supplemented with 0.9% methylcellulose, 1% bovine serum albumin, 80 micrograms/ml human transferrin, 3 micrograms/ml insulin, 2.8 micrograms/ml linoleic acid, 2.6 micrograms/ml cholesterol, 20 microM ethanolamine, and trace elements. Colony formation by WiDr cells is linear with the numbers of cells plated, having a plating efficiency (PE) of 34%, as compared to 26% in serum-containing medium. Two of the melanoma cell lines. MP6 and MeIuso, exhibit linear relationships between colony numbers and cell concentration with PEs of 21% and 70% respectively. Colony formation by the other two melanoma cell lines appears to be nonlinear. This work represents a step toward standardizing culture conditions for human tumor clonogenic cell assays.

Topics: Adenocarcinoma; Cell Aggregation; Cell Division; Cell Line; Colonic Neoplasms; Colony-Forming Units Assay; Culture Media; Humans; Melanoma; Methylcellulose; Serum Albumin, Bovine; Tumor Stem Cell Assay

The correlation of the colony growth of cells disaggregated from human melanoma, sarcoma, lung, and ovarian carcinomas were studied in four different semisolid tissue culture assays: (a) the soft agar assay of Pluznik and Sachs; (b) the soft agar assay of Hamburger and Salmon; (c) the soft agar-methyl cellulose assay of Buick et al.; and (d) the methyl cellulose assay of Ogawa et al. There was no colony growth of tumor cells achieved in 15 of 15 cases assayed in Ogawa's methyl cellulose assay. The plating efficiency of the above mentioned tumors was similar in the assays of Pluznik and Sachs, Hamburger and Salmon, and Buick et al. However, the tumor take rate differed among these three systems. The assay of Buick et al. appears potentially useful for analysis of the biology of human tumors.

Topics: Adenocarcinoma; Agar; Cell Division; Cells, Cultured; Cytological Techniques; Female; Humans; Lung Neoplasms; Melanoma; Methylcellulose; Ovarian Neoplasms; Sarcoma

A modification of the small-bowel enema with barium and methylcellulose is described and findings in 300 consecutive examinations compared with the clinical diagnoses. The radiograph was positive in 37% of cases and the clinical examination in 29%. Diseases and abnormalities of the small bowel, including motility disorders, can be diagnosed with high sensitivity and specificity using this technique.

Topics: Adenocarcinoma; Adult; Barium Sulfate; Celiac Disease; Crohn Disease; Evaluation Studies as Topic; Gastrointestinal Motility; Humans; Intestinal Diseases; Intestinal Neoplasms; Intestinal Obstruction; Intestine, Small; Methods; Methylcellulose; Radiography

A growth inhibitory effect on adenocarcinoma TA3 ascites tumors in LAF1/J mice resulted from the repeated IP administration of subtoxic doses of 3 rifamycin derivatives: rifampicin (Rif)1, dimethylbenzyldesmethylrifampicin (DMB), and rifazone-82 (R-82). A high-viscosity methylcellulose vehicle was found to be essential for obtaining a uniform drug suspension and a significant antitumor effect by the least water soluble derivatives, DMB and R-82. The more hydrophilic derivative, Rif, was found to have a comparable growth inhibitory effect on TA3 cells when prepared in 0.9% NaCl solution with or without added methylcellulose. Oral or SC drug injections did not have an antitumor effect. The results of this study point to the importance of vehicle and route of administration in chemotherapy trials with these compounds.

Topics: Adenocarcinoma; Administration, Oral; Animals; Cell Line; Injections, Intraperitoneal; Injections, Subcutaneous; Methylcellulose; Mice; Neoplasms, Experimental; Pharmaceutical Vehicles; Rifampin; Rifamycins

Topics: Adenocarcinoma; Amino Acids; Animals; Cattle; Chickens; DNA, Neoplasm; Fibrosarcoma; Lymph Nodes; Lymphoma, Non-Hodgkin; Mammary Neoplasms, Experimental; Methylcellulose; Neoplasm Transplantation; Neoplasms, Experimental; Rats; Sarcoma, Avian; Sarcoma, Experimental; Xanthines