methylamphotericin-b and Corneal-Diseases

Quantitative mycologic techniques were used to evaluate the efficacy of topical amphotericin B methyl ester in two models of yeast infection in rabbit eyes. Doses of 1%, 0.5%, and 0.15% were used in a model of superficial Candida albicans infection. The 1% dose of drug was highly efficacious, abolishing the disease after 2 days of treatment. With doses of 0.5% and 0.15%, decreasing efficacy was observed. Antifungal activity did not deteriorate when 1% prednisolone acetate was administered concomitantly with the 1% dose. In a model of deep stromal infection, the administration of topical 1% amphotericin B methyl ester was highly efficacious when the corneal epithelium was absent. Even in corneas with intact epithelium, a reduced though still significant effect was noted.

Topics: Administration, Topical; Amphotericin B; Animals; Antifungal Agents; Candidiasis; Corneal Diseases; Dose-Response Relationship, Drug; Drug Evaluation, Preclinical; Drug Therapy, Combination; Prednisolone; Rabbits; Time Factors