methyl-thiohydantoin-tryptophan and Cell-Transformation--Neoplastic

Immune escape is a crucial feature of cancer progression about which little is known. Elevation of the immunomodulatory enzyme indoleamine 2,3-dioxygenase (IDO) in tumor cells can facilitate immune escape. Not known is how IDO becomes elevated or whether IDO inhibitors will be useful for cancer treatment. Here we show that IDO is under genetic control of Bin1, which is attenuated in many human malignancies. Mouse knockout studies indicate that Bin1 loss elevates the STAT1- and NF-kappaB-dependent expression of IDO, driving escape of oncogenically transformed cells from T cell-dependent antitumor immunity. In MMTV-Neu mice, an established breast cancer model, we show that small-molecule inhibitors of IDO cooperate with cytotoxic agents to elicit regression of established tumors refractory to single-agent therapy. Our findings suggest that Bin1 loss promotes immune escape in cancer by deregulating IDO and that IDO inhibitors may improve responses to cancer chemotherapy.

Topics: Adaptor Proteins, Signal Transducing; Animals; Cell Transformation, Neoplastic; DNA-Binding Proteins; Drug Synergism; Enzyme Inhibitors; Indoleamine-Pyrrole 2,3,-Dioxygenase; Indoles; Interferon-gamma; Mammary Neoplasms, Experimental; Mice; Molecular Sequence Data; Nerve Tissue Proteins; NF-kappa B; Paclitaxel; Rats; STAT1 Transcription Factor; Thiohydantoins; Trans-Activators; Tryptophan Oxygenase; Tumor Escape; Tumor Suppressor Proteins