methyl-3-5-di-o-caffeoyl-quinate and Inflammation

One new octulosonic acid derivative, chrysannol A (1), along with 17 known compounds (2-18), were isolated from Chrysanthemum indicum flowers. Their structures were determined from 1D NMR, 2D NMR, HR-ESI-MS spectral data, and comparisons with previous reports. The effects of these compounds on lipopolysaccharide (LPS)-induced nitric oxide (NO) and tumor necrosis factor alpha (TNF-α) production by RAW 264.7 cells were investigated. Compound 8 showed the highest inhibition of NO production of 46.09% at a concentration of 10.0μM. Compounds 7, 10, 11, and 16 inhibited TNF-α secretion at all concentration tested (0.4, 2.0, and 10.0μM), with inhibition values ranging from 22.27% to 33.13%. In addition, compound 8 and 9 decrease COX-2 and iNOS protein on Western blot analysis in dose dependent manner.

Topics: Anti-Inflammatory Agents; Cells, Cultured; Chrysanthemum; Cyclooxygenase 2; Flavonoids; Flowers; Inflammation; Lipopolysaccharides; Macrophages; Magnetic Resonance Spectroscopy; Models, Molecular; Molecular Structure; Nitric Oxide; Nitric Oxide Synthase Type II; Phytotherapy; Plant Extracts; Spectrometry, Mass, Electrospray Ionization; Structure-Activity Relationship; Tumor Necrosis Factor-alpha