methoxysuccinyl-alanyl-alanyl-prolyl-valine-chloromethyl-ketone and Hemorrhage

methoxysuccinyl-alanyl-alanyl-prolyl-valine-chloromethyl-ketone has been researched along with Hemorrhage* in 2 studies

Other Studies

2 other study(ies) available for methoxysuccinyl-alanyl-alanyl-prolyl-valine-chloromethyl-ketone and Hemorrhage

ArticleYear
An inhibitor of leukocyte elastase prevents immune complex-mediated hemorrhage in the rat lung.
    The Journal of pharmacology and experimental therapeutics, 1995, Volume: 274, Issue:1

    The typical reverse passive Arthus reaction (RPA) was attained in rats by the instillation of a rabbit antiovalbumin serum into the lungs and intravenous injection of ovalbumin. Instillation of antiserum alone caused accumulation of polymorphonuclear leukocytes (PMN) and increased vascular permeability, but did not cause hemorrhage. However, when an intravenous injection of ovalbumin was also given, the vascular permeability of the lungs increased dramatically and PMN, as well as hemoglobin, were measurable in the lung lavage fluids by 4 hr after initiation of the reaction. Various proteinase inhibitors were instilled into the lungs after the initial stages of the RPA had developed, specifically to investigate their effect on the development of the hemorrhage, which we chose to monitor as an indicator of severe vascular damage. A cephalosporin-based beta-lactam, L-658,758, which is a time-dependent inhibitor of human and rat PMN elastase, effectively prevented the lung hemorrhage associated with the RPA reaction (ED50 = 2 x 55 micrograms doses/animal when instilled at 1.5 and 2.5 hr after initiating the RPA). The PMN elastase inhibitor, methoxysuccinyl-alanyl-alanyl-prolyl-valine-chloromethylketone, also inhibited hemorrhage in this model. Compounds of the same chemical class as these elastase inhibitors, but having no activity against PMN elastase in vitro, did not affect the hemorrhage associated with the RPA. Several specific inhibitors of proteinases other than PMN elastase (e.g., pepstatin and methoxysuccinyl-prolyl-glycyl-alanyl-lysine-chloromethylketone) were found to have little effect on the hemorrhage associated with the RPA reaction.(ABSTRACT TRUNCATED AT 250 WORDS)

    Topics: Amino Acid Chloromethyl Ketones; Amino Acid Sequence; Animals; Antigen-Antibody Complex; Cephalosporins; Hemorrhage; Leukocyte Elastase; Lung Diseases; Male; Molecular Sequence Data; Neutrophils; Pancreatic Elastase; Rats

1995
A comparison of alpha 1-proteinase inhibitor methoxysuccinyl-Ala-Ala-Pro-Val-chloromethylketone and specific beta-lactam inhibitors in an acute model of human polymorphonuclear leukocyte elastase-induced lung hemorrhage in the hamster.
    The American review of respiratory disease, 1990, Volume: 141, Issue:3

    A pharmacokinetic model is described for testing of polymorphonuclear leukocyte (PMN) elastase inhibitors administered by intratracheal or aerosol dosing of hamsters. Acute lung injury, measured as hemorrhage occurring within hours after intratracheal instillation of human PMN elastase, correlated directly with the amount of active enzyme instilled. Hemorrhage began within minutes of elastase instillation, was maximal within 1 h, and remained constant for up to 5 h subsequently. Therefore, inhibition of hemorrhage was used as an assay of the effectiveness of various PMN elastase inhibitors given by the intratracheal route. Lung hemorrhage could also be induced by intratracheal instillation of other elastolytic enzymes, such as thermolysin, and inhibition of hemorrhage was seen only with inhibitors active against the type of elastase used. Methoxysuccinyl-alanyl-alanyl-prolyl-valine-chloromethylketone (MeOSuc-AAPV-CMK), as well as alpha 1-proteinase inhibitor (alpha 1PI) but not tosyl-lysine-chloromethylketone (tosyl-lysine-CMK), inhibited the hemorrhage caused by human PMN elastase, but the specific inhibitors of this enzyme had no effect on thermolysin-induced lung hemorrhage. The duration of activity of these compounds as elastase inhibitors in this model correlated directly with the extent of their persistence in lung lavage fluid as determined by HPLC analysis of compound recovered by bronchoalveolar lavage. (ABSTRACT TRUNCATED AT 250 WORDS)

    Topics: alpha 1-Antitrypsin; Amino Acid Chloromethyl Ketones; Animals; Anti-Bacterial Agents; beta-Lactams; Bronchoalveolar Lavage Fluid; Cricetinae; Disease Models, Animal; Hemorrhage; Humans; Instillation, Drug; Lung Diseases; Male; Mesocricetus; Neutrophils; Pancreatic Elastase; Thermolysin; Trachea

1990