metallothionein and Pulmonary-Disease--Chronic-Obstructive

metallothionein has been researched along with Pulmonary-Disease--Chronic-Obstructive* in 2 studies

Other Studies

2 other study(ies) available for metallothionein and Pulmonary-Disease--Chronic-Obstructive

ArticleYear
Exacerbation of Elastase-Induced Emphysema via Increased Oxidative Stress in Metallothionein-Knockout Mice.
    Biomolecules, 2022, 04-15, Volume: 12, Issue:4

    Although the pathogenesis of chronic obstructive pulmonary disease (COPD) is not yet fully understood, recent studies suggest that the disruption of the intracellular balance of oxidative (such as reactive oxygen species (ROS)) and antioxidant molecules plays an important role in COPD development and progression. Metallothionein is an endogenous metal-binding protein with reported ROS scavenging activity. Although there have been many publications on the protective effects of metallothionein in the kidney and liver, its role in COPD models such as elastase- or cigarette smoke (CS)-induced lung injury is unknown. Thus, in the present study, we analyzed the elastase-induced lung injury model using metallothionein-knockout (MT-KO; MT-1 and -2 gene deletion) mice. The expression of MT-1 and MT-2 in the lungs of MT-KO mice was markedly lower compared with that in the lungs of wildtype (WT) mice. Porcine pancreatic elastase (PPE)-induced lung injury (alveolar enlargement and respiratory impairment) was significantly exacerbated in MT-KO mice compared with WT mice. Additionally, PPE-induced increases in the number of inflammatory cells, inflammatory cytokines, and cell death in lung tissue were significantly more pronounced in MT-KO mice compared with WT mice. Finally, using an in vivo imaging system, we also found that PPE-induced ROS production in the lungs was enhanced in MT-KO mice compared with WT mice. These results suggest that metallothionein may act as an inhibitor against elastase-induced lung injury by suppressing ROS production. These results suggest that metallothionein protein, or compounds that can induce metallothionein, could be useful in the treatment of COPD.

    Topics: Animals; Emphysema; Lung Injury; Metallothionein; Mice; Mice, Knockout; Oxidative Stress; Pancreatic Elastase; Pulmonary Disease, Chronic Obstructive; Pulmonary Emphysema; Reactive Oxygen Species; Swine

2022
Cadmium attenuates the macrophage response to LPS through inhibition of the NF-κB pathway.
    American journal of physiology. Lung cellular and molecular physiology, 2016, Oct-01, Volume: 311, Issue:4

    Chronic obstructive pulmonary disease (COPD) in the U.S. is primarily caused by cigarette smoking. COPD patients are highly susceptible to respiratory infections in part due to alveolar macrophage dysfunction despite a substantial increase in macrophages in the lung. Cadmium (Cd) is a toxic metal that is concentrated within tobacco and accumulates in the lung of smokers. We hypothesized that Cd uptake into macrophages alters immune function thereby impairing the macrophage response to invading pathogens. Our hypothesis was tested by comparing primary human monocytes and macrophages, primary mouse bronchoalveolar lavage myeloid cells, and related cell lines. Strikingly, Cd exposure followed by LPS stimulation resulted in a dose-dependent, significant decrease in nuclear p65 activity in macrophages that was not observed in monocytes. This corresponded with Cd-mediated inhibition of IKKβ and an impaired ability to transcribe and release cytokines in response to LPS challenge in vivo. These findings provide novel evidence that Cd has the capacity to disrupt macrophage immune function compared with monocytes. Importantly, Cd results in immune dysfunction in macrophages through inhibition of the NF-κB signaling pathway. Based on these findings, we provide new evidence that Cd contributes to immune dysfunction in the lung of COPD subjects and may increase susceptibility to infection.

    Topics: Animals; Cadmium; Cell Line; Cell Polarity; Cytokines; Female; Humans; Lipopolysaccharides; Macrophages, Alveolar; Metallothionein; Mice, Inbred C57BL; NF-kappa B; Pulmonary Disease, Chronic Obstructive; Signal Transduction; Smoking; Transcriptional Activation

2016