metallothionein has been researched along with Escherichia-coli-Infections* in 3 studies
3 other study(ies) available for metallothionein and Escherichia-coli-Infections
| Article | Year |
|---|---|
Enterotoxigenic Escherichia coli Heat-Stable Toxin Increases the Rate of Zinc Release from Metallothionein and Is a Zinc- and Iron-Binding Peptide.
Topics: Animals; Bacterial Toxins; Cell Line; Enterotoxigenic Escherichia coli; Enterotoxins; Epithelial Cells; Escherichia coli Infections; Escherichia coli Proteins; Glutathione; Host-Pathogen Interactions; Humans; Iron; Metallothionein; Peptides; Protein Binding; Swine; Zinc | 2020 |
Simultaneous analysis of host and pathogen interactions during an in vivo infection reveals local induction of host acute phase response proteins, a novel bacterial stress response, and evidence of a host-imposed metal ion limited environment.
A fundamental goal in the study of infections is to understand the dynamic interplay between host and pathogen; however, direct in vivo interrogation of this disease process via transcriptional profiling has been lacking. Here we describe the development and application of novel bacterial RNA amplification technology to simultaneously identify key elements of both host and pathogen responses in a murine infection model. On the bacterial side, we found induction of an unusual pattern of stress response genes, a response to host-induced metal ion limitation, and a failure to achieve stationary phase in vivo. On the mammalian side, we observed the surprising induction of several genes encoding acute phase response proteins including hepcidin, haptoglobin, complement C3 and metallothionein 1 at the site of infection, as well as other mediators of innate immunity. Thus, our results reveal host-pathogen cross-talk not predicted by previous in vitro analyses and provide the framework to eavesdrop on a broad array of host-pathogen interactions in vivo. As described here, the comprehensive examination of host-pathogen interactions during an infection is critical to the discovery of novel approaches for intervention not predicted by current models. Topics: Acute-Phase Proteins; Animals; Antimicrobial Cationic Peptides; Bacterial Proteins; Complement C3; Escherichia coli; Escherichia coli Infections; Female; Gene Expression Profiling; Gene Expression Regulation; Gene Expression Regulation, Bacterial; Genes; Genes, Bacterial; Granuloma; Haptoglobins; Hepcidins; Humans; Metallothionein; Metals; Mice; Mice, Inbred BALB C; Oligonucleotide Array Sequence Analysis; Oxidative Stress; Regulon; RNA, Bacterial | 2004 |
Effect of inflammatory agents and interleukin 1 on iron and zinc metabolism.
Injection of either sheep red blood cells (SRBC), Escherichia coli, endotoxin, starch, or Sephadex into chicks significantly decreased plasma iron and zinc concentrations. After injection of E. coli or SRBC, plasma zinc was decreased maximally by 52% at 12 h and 15.7% at 48 h, respectively. A second exposure to SRBC or E. coli did not further decrease plasma iron or zinc. Injection of a crude preparation of interleukin 1 isolated from endotoxin-stimulated peritoneal monocytes induced hypozincemia, hypoferremia, increased the concentration of liver metallothionein, and increased liver-zinc concentrations. It is proposed that interleukin 1 mediates the changes in trace mineral metabolism that occur during inflammation and that loss of zinc from plasma is due in part to a sequestration of zinc by liver. Topics: Animals; Chickens; Dextrans; Endotoxins; Erythrocytes; Escherichia coli Infections; Gels; Inflammation; Interleukin-1; Iron; Liver; Metallothionein; Monocytes; Starch; Zinc | 1984 |