metallothionein and Burns--Chemical

metallothionein has been researched along with Burns--Chemical* in 1 studies

Other Studies

1 other study(ies) available for metallothionein and Burns--Chemical

Article	Year
Musashi-1 post-transcriptionally enhances phosphotyrosine-binding domain-containing m-Numb protein expression in regenerating gastric mucosa. PloS one, 2013, Volume: 8, Issue:1 Upregulation of the RNA-binding protein Musashi-1 (Msi1) has been shown to occur in rat gastric corpus mucosa after ethanol-induced mucosal injury. However, there is no direct evidence linking Msi1 with gastric regeneration. We examined the process of tissue repair after acute gastric mucosal injury with Msi1-knock-out (KO) mice to clarify the role of Msi1 and Msi1-dependent regulation of m-Numb expression in regenerating gastric mucosa.. Acute gastric injury was induced in Msi1-KO and wild-type ICR mice by administering absolute ethanol. Expression of the splicing variants of m-Numb mRNA and protein in the gastric mucosa were analyzed by quantitative RT-PCR and western blotting, respectively.. We demonstrated that phosphotyrosine-binding domain-containing m-Numb expression was significantly upregulated at both the mRNA and protein levels in wild-type mice at 3 h after ethanol-induced acute gastric injury. In contrast, in Msi1-KO mice, the m-Numb protein was expressed weakly, and was associated with delayed regeneration of the injured gastric mucosal epithelium. In the Msi1-KO mouse, the ratio of m-Numb mRNA to total m-Numb mRNA in the heavy polysome fractions was lower than that in the wild-type mouse. Further, we showed that m-Numb-enhancement in gastric mucous cells induced the expression of prostate stem cell antigen and metallothionein-2. Under the m-Numb enhancing condition, the gastric cells exhibited enhanced cell proliferation and were significantly more resistant to H(2)O(2)-induced cell death than control cells.. Msi1-dependent post-transcriptional enhancement of m-Numb is crucial in gastric epithelial regeneration. Topics: Alternative Splicing; Animals; Antigens, Neoplasm; Burns, Chemical; Epithelial Cells; Ethanol; Gastric Mucosa; Gene Expression Regulation; GPI-Linked Proteins; Hydrogen Peroxide; Male; Membrane Proteins; Metallothionein; Mice; Mice, Knockout; Neoplasm Proteins; Nerve Tissue Proteins; Phosphotyrosine; Polyribosomes; Protein Structure, Tertiary; Re-Epithelialization; RNA-Binding Proteins; RNA, Messenger; Signal Transduction	2013

Article

Year

Musashi-1 post-transcriptionally enhances phosphotyrosine-binding domain-containing m-Numb protein expression in regenerating gastric mucosa.

PloS one, 2013, Volume: 8, Issue:1

Upregulation of the RNA-binding protein Musashi-1 (Msi1) has been shown to occur in rat gastric corpus mucosa after ethanol-induced mucosal injury. However, there is no direct evidence linking Msi1 with gastric regeneration. We examined the process of tissue repair after acute gastric mucosal injury with Msi1-knock-out (KO) mice to clarify the role of Msi1 and Msi1-dependent regulation of m-Numb expression in regenerating gastric mucosa.. Acute gastric injury was induced in Msi1-KO and wild-type ICR mice by administering absolute ethanol. Expression of the splicing variants of m-Numb mRNA and protein in the gastric mucosa were analyzed by quantitative RT-PCR and western blotting, respectively.. We demonstrated that phosphotyrosine-binding domain-containing m-Numb expression was significantly upregulated at both the mRNA and protein levels in wild-type mice at 3 h after ethanol-induced acute gastric injury. In contrast, in Msi1-KO mice, the m-Numb protein was expressed weakly, and was associated with delayed regeneration of the injured gastric mucosal epithelium. In the Msi1-KO mouse, the ratio of m-Numb mRNA to total m-Numb mRNA in the heavy polysome fractions was lower than that in the wild-type mouse. Further, we showed that m-Numb-enhancement in gastric mucous cells induced the expression of prostate stem cell antigen and metallothionein-2. Under the m-Numb enhancing condition, the gastric cells exhibited enhanced cell proliferation and were significantly more resistant to H(2)O(2)-induced cell death than control cells.. Msi1-dependent post-transcriptional enhancement of m-Numb is crucial in gastric epithelial regeneration.

Topics: Alternative Splicing; Animals; Antigens, Neoplasm; Burns, Chemical; Epithelial Cells; Ethanol; Gastric Mucosa; Gene Expression Regulation; GPI-Linked Proteins; Hydrogen Peroxide; Male; Membrane Proteins; Metallothionein; Mice; Mice, Knockout; Neoplasm Proteins; Nerve Tissue Proteins; Phosphotyrosine; Polyribosomes; Protein Structure, Tertiary; Re-Epithelialization; RNA-Binding Proteins; RNA, Messenger; Signal Transduction

2013