merocyanine-dye and Adenocarcinoma

merocyanine-dye has been researched along with Adenocarcinoma* in 2 studies

Other Studies

2 other study(ies) available for merocyanine-dye and Adenocarcinoma

Article	Year
pH, serum proteins and ionic strength influence the uptake of merocyanine 540 by WiDr cells and its interaction with membrane structures. Bioelectrochemistry (Amsterdam, Netherlands), 2003, Volume: 59, Issue:1-2 It has been suggested that selective uptake of photosensitizers is due to significantly lower pH of the interstitial fluid in tumors compared to normal tissue. Therefore, the cellular uptake of merocyanine 540 (MC 540) was examined at two pH values: 6.8+/-0.1 and 7.4+/-0.1. There was no difference in spectral properties (absorption and fluorescence maxima positions, fluorescence intensity) of the drug in the presence of increasing amounts of either human blood plasma or FCS (0-2%) at the two pH values investigated. Nevertheless, significantly higher amounts of the drug were taken up by WiDr cells at pH 6.8+/-0.1, both in the presence of 10% FCS and in the absence of FCS. The absorption spectra of MC 540 in the presence of egg phosphatidylcholine (PC) liposomes turned out to be NaCl concentration-dependent (0.00-0.30 mol l(-1)). Membrane fluidity, as measured by fluorescence anisotropy of diphenylhexatriene (DPH), was unchanged within the experimental error in the NaCl concentration range 0.01-0.30 mol l(-1). The spectral changes indicated an enhancement of the incorporation of MC 540 into lipid membranes with increasing ionic strength. Such a salt concentration dependence suggests a possible involvement of the surface potential in the interaction of MC 540 with lipid membranes. The results might provide an explanation of the pH dependency of the cellular uptake of MC 540 observed in this study. Topics: Adenocarcinoma; Blood Proteins; Cell Membrane Structures; Colonic Neoplasms; Culture Media; Humans; Hydrogen-Ion Concentration; Ions; Lipid Bilayers; Liposomes; Photosensitizing Agents; Pyrimidinones; Spectrometry, Fluorescence; Tumor Cells, Cultured	2003
Laser-assisted microinjection into targeted animal cells. Biotechnology letters, 2003, Volume: 25, Issue:11 A pulsed (17 nanoseconds) Nd:YAG laser (1064 nm) was used to inject impermeable dyes (propidium iodide andiodide and merocyanine 540) and a plasmid (pEGFP-N1) encoding green fluorescent protein (GFP) into human breast adenocarcinoma cells (MCF-7). The cell membrane integrity and viability were fully preserved in this laser-assisted transfer. Topics: Adenocarcinoma; Breast Neoplasms; Cell Membrane Permeability; Cell Survival; Drug Delivery Systems; Green Fluorescent Proteins; Humans; Lasers; Luminescent Proteins; Microinjections; Plasmids; Propidium; Pyrimidinones; Transfection; Transformation, Genetic; Tumor Cells, Cultured	2003

Article

Year

pH, serum proteins and ionic strength influence the uptake of merocyanine 540 by WiDr cells and its interaction with membrane structures.

Bioelectrochemistry (Amsterdam, Netherlands), 2003, Volume: 59, Issue:1-2

It has been suggested that selective uptake of photosensitizers is due to significantly lower pH of the interstitial fluid in tumors compared to normal tissue. Therefore, the cellular uptake of merocyanine 540 (MC 540) was examined at two pH values: 6.8+/-0.1 and 7.4+/-0.1. There was no difference in spectral properties (absorption and fluorescence maxima positions, fluorescence intensity) of the drug in the presence of increasing amounts of either human blood plasma or FCS (0-2%) at the two pH values investigated. Nevertheless, significantly higher amounts of the drug were taken up by WiDr cells at pH 6.8+/-0.1, both in the presence of 10% FCS and in the absence of FCS. The absorption spectra of MC 540 in the presence of egg phosphatidylcholine (PC) liposomes turned out to be NaCl concentration-dependent (0.00-0.30 mol l(-1)). Membrane fluidity, as measured by fluorescence anisotropy of diphenylhexatriene (DPH), was unchanged within the experimental error in the NaCl concentration range 0.01-0.30 mol l(-1). The spectral changes indicated an enhancement of the incorporation of MC 540 into lipid membranes with increasing ionic strength. Such a salt concentration dependence suggests a possible involvement of the surface potential in the interaction of MC 540 with lipid membranes. The results might provide an explanation of the pH dependency of the cellular uptake of MC 540 observed in this study.

Topics: Adenocarcinoma; Blood Proteins; Cell Membrane Structures; Colonic Neoplasms; Culture Media; Humans; Hydrogen-Ion Concentration; Ions; Lipid Bilayers; Liposomes; Photosensitizing Agents; Pyrimidinones; Spectrometry, Fluorescence; Tumor Cells, Cultured

2003

Laser-assisted microinjection into targeted animal cells.

Biotechnology letters, 2003, Volume: 25, Issue:11

A pulsed (17 nanoseconds) Nd:YAG laser (1064 nm) was used to inject impermeable dyes (propidium iodide andiodide and merocyanine 540) and a plasmid (pEGFP-N1) encoding green fluorescent protein (GFP) into human breast adenocarcinoma cells (MCF-7). The cell membrane integrity and viability were fully preserved in this laser-assisted transfer.

Topics: Adenocarcinoma; Breast Neoplasms; Cell Membrane Permeability; Cell Survival; Drug Delivery Systems; Green Fluorescent Proteins; Humans; Lasers; Luminescent Proteins; Microinjections; Plasmids; Propidium; Pyrimidinones; Transfection; Transformation, Genetic; Tumor Cells, Cultured

2003