mercaptopurine and Serum-Sickness

Acute glomerulonephritis characterized by proteinuria, hypoalbuminemia and leukocytosis was induced in mice by repeated intraperitoneal injections of calf serum (1 ml/mouse X 10). In mice treated with calf serum, hypercellularity, karyorrhexis, expansion of the mesangium and hyalinosis in the glomeruli were observed by light microscopy. Furthermore, circulating immune complexes were detected in the serum, and deposits of mouse IgG and C3 on the basement membranes of the glomeruli were demonstrated immunohistochemically. Oral administration of cyclophosphamide or 6-mercaptopurine at a dose of 20 mg/kg/day significantly suppressed the development of this nephritis. Dexamethasone (0.5 mg/kg/day) caused moderate inhibition of the nephritic changes. These results suggest that this experimental model may be useful for evaluation of anti-nephritic drugs.

Topics: Animals; Anti-Inflammatory Agents; Blood Cell Count; Body Weight; Cyclophosphamide; Dexamethasone; Disease Models, Animal; Fluorescent Antibody Technique; Immunochemistry; Indomethacin; Kidney Glomerulus; Male; Mercaptopurine; Mice; Mice, Inbred ICR; Nephritis; Organ Size; Serum Sickness

Topics: Aged; Animals; Antibodies; Antibody Formation; Anticoagulants; Azathioprine; Blood Transfusion; Cattle; Child; Cyclophosphamide; Elbow; Factor VIII; Female; Gastrointestinal Hemorrhage; Hemarthrosis; Hematoma; Hemophilia A; Humans; Immunosuppressive Agents; Knee; Male; Mercaptopurine; Methotrexate; Middle Aged; Penicillin G Benzathine; Prednisone; Serum Sickness; Swine; Thigh