mercaptopurine and Sarcoma-180

Topics: Animals; Antineoplastic Agents; Cardiotoxicity; Cisplatin; Drug Carriers; Drug Compounding; Kidney; Kinetics; Liver; Male; Mercaptopurine; Mice, Inbred ICR; Microscopy, Electron, Transmission; Myocardium; Nanoparticles; Nanotechnology; Porosity; Sarcoma 180; Silicon Dioxide; Solubility; Technology, Pharmaceutical; Tissue Distribution; Tumor Burden

The effects of cytostatic treatment on urinary polyamine excretion have been investigated in tumor-bearing (either Ehrlich carcinoma of S 180 sarcoma) and in tumor-free mice. The animals were exposed to single or multiple treatment with various doses of cyclophosphamide, 5-fluorouracil, or 6-mercaptopurine. Treatment invariably enhanced polyamine excretion dependent on dose and effectiveness of the cytostatic drug. The most pronounced increases were observed in the excretion of spermine and putrescine, with peak excretion usually occurring after 1-2 and 3-4 days, respectively. The urinary excretion of spermidine was relatively modest in untreated mice, but the increases observed following drug treatment were high in proportion. Significant differences in urinary polyamine excretion were observed between tumor-free and tumor-bearing animals following treatment with all cytostatic agents. Peak values were invariably higher in tumor-bearing mice even in those with small, barely detectable tumors. After discontinuation of treatment polyamine excretion returned to normal values and stabilized in groups in which regression predominated, whereas in those groups of animals which showed little or no tumor regression urinary polyamine levels gradually increased again during a 2-week observation period.

Topics: Animals; Carcinoma, Ehrlich Tumor; Creatinine; Cyclophosphamide; Female; Fluorouracil; Mercaptopurine; Mice; Neoplasm Transplantation; Polyamines; Sarcoma 180; Time Factors

Combinations of retinol palmitate (RP) and six different anticancer agents were examined to determine their effects on the life-span of mice bearing ascites sarcoma 180 or P388 leukemia. With ascites sarcoma 180, administration of a fixed dose of RP (3.3 mg/kg) considerably enhanced the antitumor effects of 5-fluorouracil (5-FU) (5 mg/kg, or 20 mg/kg), methotrexate (MTX) (0.5 mg/kg, or 1 mg/kg) and 1-(4-amino-2-methyl-5-pyrimidinyl)methyl-3-(2-chloroethyl)-3-nitrosourea (ACNU) (12.5 mg/kg), when given by intraperitoneal injection. However RP failed to potentiate the antitumor effects of adriamycin (ADM) and 6-mercaptopurine (6-MP) against sarcoma 180. With P388 leukemia, RP (167 mg/kg, or 333 mg/kg) enhanced the antitumor effects of 6-MP (25 mg/kg, or 50 mg/kg), MTX (1 mg/kg, or 2 mg/kg), ADM (0.2 mg/kg), ACNU (5 mg/kg) and cis-dichlorodiammine-platinum (CDDP) (1 mg/kg) to a considerable extent, but it did not potentiate the antitumor effect of 5-FU. The combination of RP with ACNU or CDDP was particularly effective against P388 leukemia.

Topics: Animals; Antineoplastic Combined Chemotherapy Protocols; Cisplatin; Diterpenes; Doxorubicin; Drug Evaluation, Preclinical; Drug Synergism; Fluorouracil; Leukemia P388; Male; Mercaptopurine; Methotrexate; Mice; Mice, Inbred Strains; Neoplasm Transplantation; Neoplasms, Experimental; Nimustine; Nitrosourea Compounds; Retinyl Esters; Sarcoma 180; Vitamin A

The influence of three chosen model sulfhydryl groups containing compounds, characterized by increasing size of molecule--cysteine, glutathione and insulin on antineoplastic activity of 5-fluorouracil (5-FU) and 6-mercaptopurine (6-MP) against murine leukemia L-1210 5-FU and 6-MP and murine sarcoma Sa 180, was studied. Sulfhydryl compounds failed to change in most cases the therapeutic (antineoplastic) effect of 5-FU and 6-MP. However, certain doses of these sulfhydryl compounds enhanced the cytostatic effect of 6-MP in respect to Sa-180.

Topics: Animals; Cysteine; Drug Synergism; Fluorouracil; Glutathione; Insulin; Leukemia L1210; Mercaptopurine; Mice; Mice, Inbred DBA; Neoplasms, Experimental; Sarcoma 180; Sulfhydryl Compounds

Antitumor activity and toxicity to host of newly synthesized disulfide derivatives of 6-mercaptopurine (6-MP) and 6-thioguanine (6-TG) were examined in murine ascites sarcoma-180 system (total packed cell volume method) by parenteral administration. The compounds tested were 6-alkyl disulfides (carbon number of alkyl group: 2, 3, 4, 5, 6, 7, 8, 10, and 14), 6-branched-alkyl disulfides (iso-propyl, sec-butyl, and tert-butyl), and 6-aralkyl disulfide (naphthyryl). Most disulfide derivatives of 6-MP and 6-TG showed higher antitumor activity (lower ED50) and higher toxicity to host (lower LD50) than parent compounds, but ratios of increase in activity and toxicity were different with each other. The compounds with higher chemotherapeutic index (LD50/ED50) than parent compounds were alpha-naphthyryl (8.7) disulfide in a series of 6-MP (7.5) derivatives; and sec-butyl (27), tert-butyl (24), octyl (23), decyl (26), and alpha-naphthyryl (28) disulfides in a series of 6-TG (14) derivatives. These 6-TG derivatives were promising for antitumor agents.

Topics: Animals; Antineoplastic Agents; Disulfides; Mercaptopurine; Mice; Sarcoma 180; Structure-Activity Relationship; Thioguanine

Topics: Animals; Antineoplastic Agents; Deoxyribonucleosides; Female; Mercaptopurine; Mice; Mice, Inbred ICR; Purine Nucleosides; Sarcoma 180; Sarcoma, Experimental; Thioinosine; Thionucleosides; Vidarabine; Vidarabine Phosphate

Topics: Alkaline Phosphatase; Animals; Drug Resistance; Female; Hot Temperature; Hydrogen-Ion Concentration; Isoenzymes; Mercaptopurine; Mice; Sarcoma 180; Solubility; Substrate Specificity; Thionucleotides

Topics: Alkaline Phosphatase; Animals; Cross Reactions; Drug Resistance; Epitopes; Female; Immunodiffusion; Isoenzymes; Mercaptopurine; Mice; Sarcoma 180; Tissue Distribution

Topics: Anaphylaxis; Animals; Antigens; Antineoplastic Agents; Binding Sites, Antibody; Blood Proteins; Dogs; Epitopes; Guinea Pigs; Humans; Immune Sera; Immunization, Passive; Mercaptopurine; Mice; Protein Binding; Rabbits; Sarcoma 180

Topics: Alkaline Phosphatase; Animals; Antineoplastic Agents; Carcinoma, Hepatocellular; Drug Resistance; Female; Liver Neoplasms; Mercaptopurine; Mice; Neoplasms, Experimental; Rats; Ribonucleoside Diphosphate Reductase; Sarcoma 180; Structure-Activity Relationship; Thiosemicarbazones

Topics: Animals; Antibiotics, Antineoplastic; Azaserine; Carbon Radioisotopes; Feedback; Female; Glutamine; Glycine; Mercaptopurine; Mice; Mice, Inbred Strains; Pentosephosphates; Pentosyltransferases; Phosphoric Acids; Purine Nucleosides; Purines; Pyrazoles; Pyrimidinones; Ribonucleosides; Sarcoma 180; Selenium; Spectrophotometry, Ultraviolet; Structure-Activity Relationship

Topics: Adenosine; Animals; Benzene Derivatives; Carcinoma 256, Walker; Cell Division; Cells, Cultured; Drug Synergism; Evaluation Studies as Topic; Leukemia L1210; Mercaptopurine; Mice; Mice, Inbred DBA; Rats; Sarcoma 180; Thioguanine

Topics: Animals; Azaserine; Cell Division; Cell Line; Cells, Cultured; Chromatography, Thin Layer; Drug Synergism; Female; Guanine Nucleotides; Guanosine Triphosphate; Half-Life; Mercaptopurine; Mice; Nucleosides; Nucleotides; Peritoneal Cavity; Ribonucleosides; Ribonucleotides; Sarcoma 180; Sulfur Isotopes; Thioguanine; Time Factors; Uracil Nucleotides

Topics: Acetazolamide; Animals; Antineoplastic Agents; Carcinoma, Squamous Cell; Diuretics; Female; Furosemide; Male; Melphalan; Mercaptopurine; Mice; Neoplasm Transplantation; Neoplasms, Experimental; Phosphines; Rats; Sarcoma 180; Sarcoma, Experimental; Serotonin

Topics: Adenine Nucleotides; Adenosine Triphosphate; Animals; Carbon Isotopes; Chromatography, Paper; Drug Synergism; Female; Guanine Nucleotides; Guanosine Triphosphate; Mercaptopurine; Methylation; Mice; Nucleosides; Ribonucleosides; Sarcoma 180

Topics: Adenocarcinoma; Animals; Antineoplastic Agents; Carcinoma, Ehrlich Tumor; Cell Line; Cyclization; Cyclophosphamide; Cytarabine; Cytidine; Dose-Response Relationship, Drug; Female; Fluorouracil; Leukemia L1210; Leukemia, Experimental; Male; Mercaptopurine; Methotrexate; Mice; Mitomycins; Neoplasms, Experimental; Sarcoma 180

Topics: Acetates; Animals; Antineoplastic Agents; Busulfan; Central Nervous System Stimulants; Cyclophosphamide; Drug Synergism; Fluorouracil; Meclofenoxate; Mercaptopurine; Methotrexate; Mice; Mitomycins; Sarcoma 180; Vinblastine; Vincristine

Topics: Animals; Drug Synergism; Lymphoma, Non-Hodgkin; Mercaptopurine; Mice; Neoplasm Transplantation; Neoplasms, Experimental; Sarcoma 180; Uracil

Topics: Acid Phosphatase; Alkaline Phosphatase; Animals; Calcium; Cell Line; Cell-Free System; Drug Resistance; Female; Hydrogen-Ion Concentration; In Vitro Techniques; Magnesium; Mercaptopurine; Mice; Nucleotidases; Sarcoma 180; Subcellular Fractions; Thioguanine

Effects of heating and fasting, both alone and associated, have been studied in normal and Sarcoma 180 bearing mice. Heating reduced body weight and tumour mass and increased body temperature. Fasting reduced body weight, while tumour mass and body temperature were slightly affected. By associating heating and fasting a more marked decrease of body weight was produced than by each of the two factors involved, while effects on body temperature and on tumour mass were unchanged with respect to heating alone. 6-mercaptopurine was similar to heating in reducing body weight and tumour mass.

Topics: Animals; Body Temperature; Body Weight; Fasting; Female; Hot Temperature; Mercaptopurine; Mice; Neoplasm Transplantation; Sarcoma 180

Topics: Animals; Antigen-Antibody Reactions; Antineoplastic Agents; Cyclophosphamide; Cytarabine; Female; Mechlorethamine; Mercaptopurine; Methanol; Mice; Neoplasm Transplantation; Pyridines; Sarcoma 180; Thiosemicarbazones; Transplantation, Homologous; Vitamin B 6 Deficiency

Topics: Amino Acids; Animals; Antineoplastic Agents; Dipeptides; Esters; Female; Mammary Neoplasms, Experimental; Mercaptopurine; Mice; Neoplasms, Experimental; Rats; Sarcoma; Sarcoma 180; Sarcoma, Yoshida; Triglycerides

Topics: Animals; Male; Mercaptopurine; Mice; Mice, Inbred Strains; Nucleosides; Ribonucleosides; Sarcoma 180; Sarcoma, Experimental; Thioguanine

Topics: Adenine; Adenocarcinoma; Animals; Azaserine; Carbon Isotopes; Cell Line; Feedback; Hypoxanthines; Mercaptopurine; Methotrexate; Neoplasms, Experimental; Nucleotides; Phosphotransferases; Purines; Sarcoma 180; Thioguanine

Topics: Adenocarcinoma; Alkylating Agents; Animals; Antimetabolites; Cyclophosphamide; Mercaptopurine; Mice; Mitosis; Neoplasm Transplantation; Neoplasms, Experimental; Plasmacytoma; Sarcoma 180

Topics: Animals; Disease Models, Animal; Drug Synergism; Immunization, Passive; Immunosuppressive Agents; Leukemia L1210; Mercaptopurine; Mice; Sarcoma 180

Topics: Animals; Carcinoma 256, Walker; Female; Immune Sera; Immunization; Liver; Mercaptopurine; Mice; Pyridoxine; Sarcoma 180; Spleen; Thiosemicarbazones; Thymus Gland

Topics: Animals; Antibody Formation; Carcinoma, Ehrlich Tumor; Diet Therapy; Female; Male; Mercaptopurine; Mice; Neoplasm Regression, Spontaneous; Neoplasm Transplantation; Sarcoma 180; Spleen; Splenectomy; Thiosemicarbazones; Thymectomy; Vitamin B 6 Deficiency

Topics: Animals; Antineoplastic Agents; Ascites; Carcinoma, Ehrlich Tumor; Cyclophosphamide; Electronics, Medical; Mannomustine; Melphalan; Mercaptopurine; Methods; Mice; Sarcoma 180

Topics: Animals; Animals, Newborn; Female; Immunosuppressive Agents; Male; Mercaptopurine; Mice; Neoplasm Transplantation; Sarcoma 180; Splenectomy; Thiosemicarbazones; Thymectomy; Thymus Gland; Transplantation, Homologous

Topics: Abnormalities, Drug-Induced; Animals; Azaserine; Carcinoma, Ehrlich Tumor; DNA, Neoplasm; Female; Glucosyltransferases; Leukemia L1210; Mammary Neoplasms, Experimental; Mercaptopurine; Mice; Nucleosides; Oxidoreductases; Phosphotransferases; Pregnancy; Pregnancy, Animal; Sarcoma 180; Sarcoma, Experimental

Topics: Adenocarcinoma; Alkylation; Animals; Antimetabolites; Carcinoma, Ehrlich Tumor; Glutamine; Leukemia, Experimental; Leukemia, Radiation-Induced; Mammary Neoplasms, Experimental; Mercaptopurine; Mice; Neoplasm Transplantation; Sarcoma 180

Topics: Animals; Carcinoma, Ehrlich Tumor; Female; Mercaptopurine; Mice; Neoplasm Transplantation; Neoplasms; Sarcoma 180; Thiosemicarbazones; Vitamin B 6 Deficiency

Topics: Animals; Antineoplastic Agents; Drug Synergism; Female; Leukemia L1210; Mercaptopurine; Mice; Neoplasm Transplantation; Sarcoma 180; Vitamin B 6 Deficiency

Topics: Adenine Nucleotides; Animals; Carbon Isotopes; Liver; Mercaptopurine; Metabolism; Mice; Nucleotides; Pharmacology; Radiometry; Research; Sarcoma 180

Topics: Adenine Nucleotides; Animals; Liver; Mercaptopurine; Metabolism; Mice; Neoplasms; Neoplasms, Experimental; Nucleotides; Pharmacology; Research; Sarcoma 180

Topics: Animals; Antibody Formation; Chemistry, Pharmaceutical; Injections, Intraperitoneal; Mercaptopurine; Mice; Neoplasms, Experimental; Oxides; Pharmacology; Purines; Research; Sarcoma 180; Toxicology

Topics: Animals; Anti-Bacterial Agents; Antineoplastic Agents; Genetics; Mercaptopurine; Mice; Neoplasm Transplantation; Sarcoma 180; Vitamin B 6 Deficiency; Zymosan

Topics: Animals; Anti-Bacterial Agents; Antineoplastic Agents; Azaserine; Carcinoma, Squamous Cell; Chelating Agents; Cortisone; Leucine; Mercaptopurine; Mice; Neoplasms; Neoplasms, Experimental; Rats; Research; Sarcoma 180; Streptomycin; Thioguanine; Transplantation

Topics: Animals; Antineoplastic Agents; Aspartic Acid; Azaguanine; Carbon Isotopes; Glycine; Guanine Nucleotides; Imidazoles; Liver; Lyases; Mercaptopurine; Metabolism; Mice; Neoplasms, Experimental; Nucleotides; Pharmacology; Purines; Research; Sarcoma 180; Thioguanine

Topics: Adenocarcinoma; Animals; Breast Neoplasms; Cortisone; Humans; Mammary Neoplasms, Animal; Mammary Neoplasms, Experimental; Mercaptopurine; Mice; Neoplasms; Neoplasms, Experimental; Nitrogen Mustard Compounds; Pharmacology; Puromycin; Research; Sarcoma 180; Uracil Mustard; Zymosan

Topics: Animals; Anti-Bacterial Agents; Antibiotics, Antineoplastic; Antineoplastic Agents; Busulfan; Carcinoma, Ehrlich Tumor; Leukemia; Leukemia, Myeloid; Lymphoma; Lymphoma, Non-Hodgkin; Mercaptopurine; Mice; Neoplasms, Experimental; Nitrogen Mustard Compounds; Olivomycins; Pharmacology; Research; Sarcoma 180; Thiotepa; Toxicology

Topics: Aminoimidazole Carboxamide; Animals; Antimetabolites; Imidazoles; Mercaptopurine; Pharmacology; Research; Research Design; Sarcoma 180; Tissue Culture Techniques

Topics: Alkylating Agents; Animals; Antibody Formation; Antineoplastic Agents; Azaserine; Imidazoles; Mercaptopurine; Mice; Pharmacology; Purines; Rats; Research; Sarcoma 180; Tetanus Toxoid; Thioguanine; Toxicology

Topics: Adenocarcinoma; Animals; Antineoplastic Agents; Carbonates; Chemistry, Pharmaceutical; Leukemia L1210; Mercaptopurine; Mice; Purines; Research; Sarcoma 180; Tissue Culture Techniques

Topics: Animals; Antineoplastic Agents; Carcinoma, Ehrlich Tumor; Glutaminase; Leukemia; Leukemia, Experimental; Lymphoma; Lymphoma, Non-Hodgkin; Mercaptopurine; Mice; Neoplasms; Neoplasms, Experimental; Pharmacology; Research; Sarcoma 180; Toxicology

Topics: Animals; Antineoplastic Agents; Carcinoma, Ehrlich Tumor; Heterocyclic Compounds; Mercaptopurine; Mice; Pharmacology; Quinazolines; Rats; Research; Sarcoma 180

Topics: Adenine; Amino Acids; Animals; Antimetabolites; Antineoplastic Agents; Azaserine; Carbon Isotopes; Glycine; Guanine; Mercaptopurine; Metabolism; Mice; Neoplasm Transplantation; Nucleotides; Pharmacology; Purines; Research; Sarcoma 180

Topics: Animals; Anti-Bacterial Agents; Antibodies; Antineoplastic Agents; Blood Cells; Carcinoma, Ehrlich Tumor; Cortisone; Cytarabine; Cytosine; Leukemia L1210; Leukemia, Experimental; Lymphoma; Mercaptopurine; Methotrexate; Mice; Neoplasms; Neoplasms, Experimental; Pharmacology; Research; Sarcoma 180

Topics: Animals; Buffers; Carbon Dioxide; Glyceric Acids; Glycerophosphates; Glycolysis; Hot Temperature; Hydro-Lyases; Hydrogen-Ion Concentration; Mercaptopurine; Metabolism; Mice; Nitrogen; Oxygen; Pharmacology; Phosphoglucomutase; Pyruvate Kinase; Pyruvates; Pyruvic Acid; Research; Sarcoma 180

Topics: Animals; Biosynthetic Pathways; Carbon Isotopes; Carcinoma; Carcinoma, Ehrlich Tumor; DNA; DNA, Neoplasm; Formates; Glycine; Leukemia L1210; Mercaptopurine; Metabolism; Nucleotides; Purines; Research; RNA; RNA, Neoplasm; Sarcoma 180

Topics: Animals; Feces; Mercaptopurine; Metabolism; Mice; Purines; Research; Sarcoma 180; Sulfur Isotopes; Urine

Topics: Adenocarcinoma; Animals; Antineoplastic Agents; Mercaptopurine; Mice; Neoplasms; Neoplasms, Experimental; Research; Sarcoma 180; Thioguanine; Toxicology

Topics: Alkylating Agents; Animals; Antineoplastic Agents; Azaguanine; Azaserine; Carcinoma, Ehrlich Tumor; Carcinoma, Hepatocellular; DNA; DNA, Neoplasm; Fluorouracil; Idoxuridine; Liver Neoplasms; Lymphoma; Lymphoma, Non-Hodgkin; Mercaptopurine; Mice; Neoplasms, Experimental; Nitrogen Mustard Compounds; Nucleosides; Nucleotides; Purines; Research; RNA; RNA, Neoplasm; Sarcoma 180; Thioguanine; Uracil Mustard

Topics: Animals; Anti-Bacterial Agents; Antibiotics, Antitubercular; Antineoplastic Agents; Busulfan; Cytostatic Agents; Mercaptopurine; Mice; Olivomycins; Pharmacology; Research; Sarcoma; Sarcoma 180; Thiotepa; Uracil

Topics: Animals; Anti-Bacterial Agents; Antineoplastic Agents; Cysteine; Diethylstilbestrol; Mechlorethamine; Mercaptopurine; Mice; Neoplasms; Pharmacology; Research; Sarcoma; Sarcoma 180; Sarcoma, Experimental

Topics: Animals; Antineoplastic Agents; Chemistry, Pharmaceutical; Mercaptopurine; Mice; Neoplasms; Pharmacology; Pyrimidines; Research; Sarcoma 180

Topics: Animals; Chemistry, Pharmaceutical; Mercaptopurine; Mice; Oxides; Pharmacology; Research; Sarcoma 180; Toxicology

Topics: Adenosine Triphosphatases; Animals; Fructose-Bisphosphate Aldolase; Glucose; Glycolysis; Isomerases; L-Lactate Dehydrogenase; Lactates; Lactic Acid; Mercaptopurine; Mice; Oxidoreductases; Phosphotransferases; Sarcoma 180

Combinations of 6-thioguanine and uracil mustard produced greater inhibition of the growth of sarcoma 180 than that equivalent to the sum of the inhibitory effects caused by the optimum levels of the individual drugs. This potentiation was accomplished without marked weight loss by the host. Some possible biochemical mechanisms responsible for the drug synergy are discussed.

Topics: Animals; Antineoplastic Agents; Mercaptopurine; Sarcoma 180; Thioguanine; Uracil; Uracil Mustard

Topics: Animals; DNA; Enzymes; Mercaptopurine; Mice; Nitrogen; Sarcoma 180; Sarcoma, Experimental

The partial mitotic inhibition caused by 6-mercaptopurine in tissue cultures of Crocker mouse sarcoma 180 and embryonic mouse skin is blocked by co-enzyme A. 6-Mercaptopurine and coenzyme A also have opposite effects on mitochondrial morphology. Mitochondria in cells treated with 6-mercaptopurine become thin and fragmented. Coenzyme A blocks this effect, and alone coenzyme A makes for longer and thicker mitochondria. 6-Mercaptopurine inhibits lipogenesis in embryo skin fibroblasts, and this inhibition is partly counteracted by coenzyme A, which by itself makes for a greater accumulation of lipid droplets in the cytoplasm. It is suggested that at least one part of the action by which 6-mercaptopurine decreases mitotic incidence in tissue cultures may be an interference on the part of 6-mercaptopurine, acting as an antimetabolite of coenzyme A, in mitochondrial function related to cell division.

Topics: Animals; Antimetabolites; Cell Division; Coenzyme A; Coenzymes; Cytoplasm; Fibroblasts; Mercaptopurine; Mice; Mitochondria; Mitosis; Purines; Sarcoma 180; Tissue Culture Techniques

Topics: Animals; Mercaptopurine; Mice; Purines; Sarcoma 180; Sarcoma, Experimental