mercaptopurine and Hypoxia

Diffuse alveolar hemorrhage is an uncommon and often fatal condition in children that is characterized by distinct histopathological etiologies. Herein, we discuss the case of an 11-year-old girl who presented with acute worsening of hypoxia and left-sided chest pain. The patient had lung biopsy-proven idiopathic pulmonary capillaritis and was being treated with prednisolone every alternate day, azathioprine, and hydroxychloroquine. A contrast-computed tomography (CT) scan of the chest showed an acute left lower-lobe pulmonary embolism. Negative results were obtained on a test for thrombophilia. In children, pulmonary embolism with anti-neutrophil cytoplasmic antibody-negative idiopathic pulmonary capillaritis is a rare clinical condition. The exact cause of thrombus formation in this case is unknown; however, obesity, immobility, and chronic systemic corticosteroid therapy probably played a role.

Topics: Acute Disease; Antibodies, Antineutrophil Cytoplasmic; Capillaries; Chest Pain; Child; Female; Hemorrhage; Heparin, Low-Molecular-Weight; Humans; Hydroxychloroquine; Hypoxia; Lung; Lung Diseases; Mercaptopurine; Prednisolone; Pulmonary Alveoli; Pulmonary Embolism; Treatment Outcome; Vasculitis

By means of ultraviolet and visible cytospectrophotometry, RNA and total protein content per cell was determined in perineuronal oligodendrogliocytes of spinal cord anterior horns and in ependyma cells of spinal cord central canal in Wistar rats under various experimental conditions. It was only in one experimental series that the cell kinds compared were characterized by a similar metabolic response: daily adrenaline injections for two weeks resulted in RNA accumulation both in the oligodendroglia and in the ependyma: besides, in both the kinds of spinal cord cells, no changes in RNA amount was found due to acute hypoxic hypoxia and to 6-mercaptopurine administration. In all the other experimental series (aurantin adminstraion: adrenalectomy and hydrocortison treatment; posthypoxic reparation), changes in RNA content markedly differed in the oligodendroglia and in the ependyma. The data are presented concerning the changes in protein content in the cytoplasm of spinal cord ependyma cells under the experimental conditions applied. Importance of topochemical analysis of nervous tissue structures by means of quantitative cytochemical methods is outlined.

Topics: Adrenalectomy; Animals; Ependyma; Epinephrine; Histocytochemistry; Hydrocortisone; Hypoxia; Male; Mercaptopurine; Nerve Tissue Proteins; Neuroglia; Oligodendroglia; Photometry; Rats; RNA; Spinal Cord