maxadilan-protein--insect has been researched along with Erythema* in 2 studies
2 other study(ies) available for maxadilan-protein--insect and Erythema
| Article | Year |
|---|---|
Maxadilan. Cloning and functional expression of the gene encoding this potent vasodilator peptide.
Maxadilan is a potent vasodilator peptide released into the skin when the sand fly Lutzomyia longipalpis, an important vector of leishmania, probes for a blood meal. As several lines of evidence suggest that this peptide may play a critical role in the enhancement of leishmania infectivity attributed to sand fly saliva, the peptide has been proposed as a candidate antigen for a leishmanial vaccine. Although maxadilan is the most potent vasodilator peptide known and shares several properties with calcitonin gene-related peptide (CGRP), studies of its structure, physiological effects, and biological roles have been limited by the miniscule quantities available. Here we report the isolation of cDNA and genomic DNA clones that encode maxadilan. The predicted translation product shows no significant homology with any previously isolated proteins. The coding DNA has been expressed in Escherichia coli and the purified recombinant peptide is biologically active with a specific activity comparable to the natural peptide. Recombinant maxadilan will be useful in studies of vascular biology and could lead to novel therapeutic and prophylactic agents. Topics: Amino Acid Sequence; Animals; Calcitonin Gene-Related Peptide; Chromatography, High Pressure Liquid; Cloning, Molecular; DNA; Erythema; Escherichia coli; Gene Expression; Humans; Insect Hormones; Insect Proteins; Molecular Sequence Data; Polymerase Chain Reaction; Protein Biosynthesis; Psychodidae; Recombinant Proteins; RNA, Messenger; Sequence Homology, Nucleic Acid; Vasodilator Agents | 1992 |
Isolation of maxadilan, a potent vasodilatory peptide from the salivary glands of the sand fly Lutzomyia longipalpis.
Blood feeding by the sand fly Lutzomyia longipalpis is aided by the presence of a vasodilator in its salivary glands. This novel vasodilator has been isolated by reversed-phase high-performance liquid chromatography. Ten nanograms of the vasodilator are present in the extract of a pair of sand fly salivary glands. It has 500 times the vasodilatory activity of calcitonin gene-related peptide, previously the most potent vasodilator peptide known. This novel peptide is thus called maxadilan. Topics: Amino Acids; Animals; Calcitonin Gene-Related Peptide; Chromatography, High Pressure Liquid; Diptera; Electrophoresis; Electrophoresis, Polyacrylamide Gel; Erythema; Insect Hormones; Insect Proteins; Isoelectric Focusing; Mass Spectrometry; Rabbits; Recombinant Proteins; Salivary Glands; Vasodilator Agents | 1991 |