mart-1-antigen and Kidney-Diseases

Renal fibrosis plays a pivotal role in the development and progression of chronic kidney disease, which affects 10% of the adult population. Previously, it has been demonstrated that the cyclooxygenase-2 (COX-2)/prostaglandin (PG) system influences the progression of renal injury. Here, we evaluated the impact of butaprost, a selective EP. We studied the anti-fibrotic efficacy of butaprost using Madin-Darby Canine Kidney (MDCK) cells, mice that underwent unilateral ureteral obstruction and human precision-cut kidney slices. Fibrogenesis was evaluated on a gene and protein level by qPCR and Western blotting.. Butaprost (50 μM) reduced TGF-β-induced fibronectin (FN) expression, Smad2 phosphorylation and epithelial-mesenchymal transition in MDCK cells. In addition, treatment with 4 mg/kg/day butaprost attenuated the development of fibrosis in mice that underwent unilateral ureteral obstruction surgery, as illustrated by a reduction in the gene and protein expression of α-smooth muscle actin, FN and collagen 1A1. More importantly, a similar anti-fibrotic effect of butaprost was observed in human precision-cut kidney slices exposed to TGF-β. The mechanism of action of butaprost appeared to be a direct effect on TGF-β/Smad signalling, which was independent of the cAMP/PKA pathway.. In conclusion, this study demonstrates that stimulation of the EP

Topics: Aged; Alprostadil; Animals; Cell Line; Dogs; Epithelial-Mesenchymal Transition; Female; Fibrosis; Humans; Kidney; Kidney Diseases; Male; MART-1 Antigen; Mice; Mice, Inbred C57BL; Receptors, Prostaglandin E, EP2 Subtype; Tissue Culture Techniques; Ureteral Obstruction; Urological Agents

Diagnosis of low-grade clear cell lesions in kidney can be challenging to the pathologist in practice. There is limited information in the literature addressing the practical issues that may be encountered in this regard. Herein is presented two cases: one was an adrenal cortical nodule; the second, an intrarenal adrenal heterotopia that was assumed to be renal cell carcinoma initially. Certain red flags should prompt pathologists to investigate further; close morphological examination including immunohistochemistry may be warranted in these lesions.

Topics: Adrenal Cortex; Antigens, Neoplasm; Biomarkers; Carcinoma, Renal Cell; Choristoma; Diagnosis, Differential; Female; Humans; Kidney Diseases; Kidney Neoplasms; MART-1 Antigen; Middle Aged; Mucin-1; Neoplasm Proteins