mangostin and Leukemia

Three new pyranoxanthones, calotetrapterins A-C (

Topics: Animals; Antineoplastic Agents, Phytogenic; Calophyllum; Cell Line, Tumor; Drug Screening Assays, Antitumor; Leukemia; Magnetic Resonance Spectroscopy; Mice; Molecular Structure; Plant Bark; Spectrometry, Mass, Electrospray Ionization; Structure-Activity Relationship; Xanthones

Beta-mangostin (BM) is a xanthone-type of natural compound isolated from Cratoxylum arborescens. This study aimed to examine the apoptosis mechanisms induced by BM in a murine monomyelocytic cell line (WEHI-3) in vitro and in vivo.. A WEHI-3 cell line was used to evaluate the cytotoxicity of BM by MTT. AO/PI and Hoechst 33342 dyes, Annexin V, multiparametric cytotoxicity 3 by high content screening (HCS); cell cycle tests were used to estimate the features of apoptosis and BM effects. Caspase 3 and 9 activities, ROS, western blot for Bcl2, and Bax were detected to study the mechanism of apoptosis. BALB/c mice injected with WEHI-3 cells were used to assess the apoptotic effect of BM in vivo.. BM exerts anti-leukaemic properties in vitro and in vivo.

Topics: Animals; Antineoplastic Agents, Phytogenic; Apoptosis; Caspase 3; Caspase 9; Cell Cycle; Cell Line, Tumor; Cell Proliferation; Clusiaceae; Leukemia; Liver; Mice, Inbred BALB C; Phytotherapy; Plant Extracts; Reactive Oxygen Species; Spleen; Xanthones

Mangostin (MAG), a kind of xanthone widely used in diet and medicine, has antioxidant, anti-inflammatory, antimicrobial, and anticancer activities. On account of its antioxidant activity, MAG might protect cancer cells from free radical damage in photodynamic therapy (PDT) during which reactive oxygen species production was stimulated leading to irreversible tumor cell injury. In this study, the antioxidant activity of MAG was investigated and the influence of MAG on K562 cells in 5-aminolevulinic acid (ALA)-based PDT is demonstrated. The results showed that MAG could scavenge hydroxyl radical, superoxide anion, and hydrogen peroxide and inhibit the formation of malondialdehyde (MDA), but increase the amounts of singlet oxygen in cell-free systems. MAG inhibits cell proliferation and enhances cell apoptosis, lipid peroxidation, and DNA damage in ALA-PDT on K562 cells. NaN3, a singlet oxygen quencher, suppresses the MAG-induced cell apoptosis, lipid peroxidation, and DNA damage. In conclusion, MAG enhances the PDT-induced cytotoxicity in K562 cells and singlet oxygen was involved in this process. These results implied that the effect of antioxidants on PDT might be determined by its sensitization ability to singlet oxygen.

Topics: Antioxidants; Apoptosis; Cell-Free System; DNA Damage; Humans; K562 Cells; Leukemia; Lipid Peroxidation; Photochemotherapy; Reactive Oxygen Species; Sodium Azide; Xanthones

We examined the effects of six xanthones from the pericarps of mangosteen, Garcinia mangostana, on the cell growth inhibition of human leukemia cell line HL60. All xanthones displayed growth inhibitory effects. Among them, alpha-mangostin showed complete inhibition at 10 microM through the induction of apoptosis.

Topics: Apoptosis; Blotting, Western; Garcinia; HL-60 Cells; Humans; Indonesia; Inhibitory Concentration 50; Leukemia; Molecular Structure; Plants, Medicinal; Stereoisomerism; Tumor Cells, Cultured; Xanthones