maltodextrin and Prostatic-Neoplasms

ID4, a helix loop helix transcriptional regulator has emerged as a tumor suppressor in prostate cancer. Epigenetic silencing of ID4 promotes prostate cancer whereas ectopic expression in prostate cancer cell lines blocks cancer phenotype. To directly investigate the anti-tumor property, full length human recombinant ID4 encapsulated in biodegradable Polycaprolactone/Maltodextrin (PCL-MD) nano-carrier was delivered to LNCaP cells in which the native ID4 was stably silenced (LNCaP(-)ID4). The cellular uptake of ID4 resulted in increased apoptosis, decreased proliferation and colony formation. Intratumoral delivery of PCL-MD ID4 into growing LNCaP(-)ID4 tumors in SCID mice significantly reduced the tumor volume compared to the tumors treated with chemotherapeutic Docetaxel. The study supports the feasibility of using nano-carrier encapsulated ID4 protein as a therapeutic. Mechanistically, ID4 may assimilate multiple regulatory pathways for example epigenetic re-programming, integration of multiple AR co-regulators or signaling pathways resulting in tumor suppressor activity of ID4.

Topics: Animals; Antineoplastic Agents; Apoptosis; Cell Line, Tumor; Cell Proliferation; Docetaxel; Drug Carriers; Humans; Inhibitor of Differentiation Proteins; Male; Mice, SCID; Nanoparticles; Polyesters; Polysaccharides; Prostatic Neoplasms; RNA Interference; Taxoids; Tumor Burden; Xenograft Model Antitumor Assays

Polysaccharide nanoparticles were studied as drug delivery vehicles for chemopreventive agents.. Green tea polyphenol epigallocatechin-3-gallate (EGCG) was incorporated into a carbohydrate matrix of gum arabic and maltodextrin with an encapsulation efficiency of approximately 85%.. Encapsulated EGCG retained its biological activity, reducing the cell viability and inducing apoptosis of Du145 prostate cancer cells. Clonogenic assay demonstrated that encapsulation of EGCG enhanced its inhibitory effect on cell proliferation (10-20%) at lower concentrations (1-2 µM), compared with free EGCG.. This study highlights the use of polysaccharide nanoparticles in chemoprevention as they can be used to deliver natural antioxidants capable of inhibiting steps of the tumorigenesis process.

Topics: Anticarcinogenic Agents; Apoptosis; Carcinoma; Caspase 3; Catechin; Cell Line; Cell Survival; Chemoprevention; Gum Arabic; Humans; Male; Nanoparticles; Polysaccharides; Prostatic Neoplasms; Tea