malonyl-coenzyme-a and Alzheimer-Disease

We evaluated the activities of carnitine palmitoyltransferase (CPT), carnitine octanoyltransferase (COT), and carnitine acetyltransferase (CAT) in the frontal cortex, temporal cortex, parietal cortex, hippocampus, and cerebellum of Alzheimer disease (AD) patients and normal human brains. There were no significant differences in total CPT activity, its inhibition by malonyl-CoA, the effect of the detergent Triton X-100 on CPT activity, COT activity, and CAT activity in any of the brain regions examined whether activities were expressed as grams of wet weight or corrected for noncollagen protein content. The addition of Triton X-100 increased CAT activity by 50%. Our results suggest that there is no defect of fatty acid transport within the AD brain cell. Total CPT activity, COT activity, and CAT activity are not affected in AD nor is the ratio of CPT I to CPT II altered in the AD versus the normal human brain.

Topics: Aged; Aged, 80 and over; Alzheimer Disease; Autopsy; Biological Transport, Active; Brain; Carnitine Acyltransferases; Carnitine O-Acetyltransferase; Carnitine O-Palmitoyltransferase; Case-Control Studies; Detergents; Enzyme Inhibitors; Fatty Acids; Female; Humans; Male; Malonyl Coenzyme A; Middle Aged; Octoxynol