malealdehyde and Uveitis

The purpose of the present study was to investigate the antibacterial and anti-inflammatory activity of Angelica sinensis extract (AE), Sophora flavescens extract (SE), and herb pair A. sinensis and S. flavescens extract (HPE). Endotoxin-induced uveitis (EIU) was induced in rats by a footpad injection of lipopolysaccharide. The anti-inflammatory potential of AE, SE, and HPE in the regulation of nuclear factor kappa B (NF-κB), maleic dialdehyde (MDA), polymorphonuclear cells (PMN), interleukin-1β (IL-1β), inducible nitric oxide synthase (iNOS) and tumor necrosis factor-α (TNF-α), adhesion molecule (ICAM-1), and cyclooxygenase-2 (COX-2) was determined by ELISA and immunohistochemistry. HPE showed strong antibacterial activity at all tested concentrations (1.25, 2.5, and 5 μg/ml) to Escherichia coli, Staphylococcus aureus, and Shigella Castellani and Chalmers. HPE significantly inhibited EIU-induced upregulation of NF-κB activation and the production of IL-1β, TNF-α, iNOS, ICAM-1, and COX-2. Moreover, HPE suppressed MDA and infiltration of PMN. The study supports the hypothesis that the antipimple and anti-eczema activities of Dangguikushen compound recipe are attributed to herb pairs, A. sinensis and S. flavescens, used in combination.

Topics: Aldehydes; Angelica sinensis; Animals; Anti-Bacterial Agents; Anti-Inflammatory Agents; Bacteria; Cyclooxygenase 2; Drug Combinations; Escherichia coli; Intercellular Adhesion Molecule-1; Interleukin-1beta; Lipopolysaccharides; Male; Microbial Sensitivity Tests; Neutrophils; NF-kappa B; Nitric Oxide Synthase Type II; Plant Extracts; Rats; Rats, Wistar; Shigella; Sophora; Staphylococcus aureus; Tumor Necrosis Factor-alpha; Uveitis