magnosalin and Granuloma

Inhibitory effects of magnosalin and magnoshinin, compounds from the crude drug "Shin-i" (Flos magnoliae), on angiogenesis and pouch granuloma formation in mice induced by an adjuvant containing croton oil were investigated. The anti-chronic inflammatory effect of "shin-i" was caused by selective inhibition of angiogenesis by magnosalin and of granuloma formation by magnoshinin.

Topics: Animals; Anti-Inflammatory Agents, Non-Steroidal; Cyclobutanes; Freund's Adjuvant; Granuloma; Hydrocortisone; Lignans; Male; Mice; Mice, Inbred Strains; Naphthols; Neovascularization, Pathologic; Plants, Medicinal

Inhibitory effects of magnosalin and magnoshinin, compounds from the crude drug 'Shin-i' (Flos magnoliae), on angiogenesis and pouch granuloma formation induced by an adjuvant containing croton oil were investigated. Magnosalin inhibited angiogenesis 2.4-fold (intra-pouch) and 9.7-fold (intraperitoneal) more strongly than granuloma formation. The inhibition of angiogenesis by magnosalin was 5-fold (intra-pouch) and 21-fold (intraperitoneal) weaker than that by hydrocortisone. In contrast, intraperitoneal magnoshinin inhibited granuloma formation 2.5-fold more strongly than angiogenesis. The regression coefficients of anti-angiogenesis vs. the inhibition of granuloma formation were 1.79 for magnosalin, 1.11 for hydrocortisone, and 0.61 for magnoshinin. These results show that the anti-chronic inflammatory effect of 'Shin-i' was caused by selective inhibition of angiogenesis by magnosalin and of granuloma formation by magnoshinin.

Topics: Adjuvants, Immunologic; Animals; Anti-Inflammatory Agents, Non-Steroidal; Cyclobutanes; Granuloma; Injections, Intraperitoneal; Lignans; Male; Mice; Naphthols; Neovascularization, Pathologic; Peritoneal Diseases; Peritoneum; Plants, Medicinal; Skin; Skin Diseases