magainin-2-peptide--xenopus and Leukemia--Basophilic--Acute
magainin-2-peptide--xenopus has been researched along with Leukemia--Basophilic--Acute* in 1 studies
Other Studies
1 other study(ies) available for magainin-2-peptide--xenopus and Leukemia--Basophilic--Acute
Article | Year |
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Magainin-2 releases histamine from rat mast cells.
The magainins are basic 23 amino acid peptides with a broad spectrum of antimicrobial activity. Their bactericidal effect has been attributed to their capacity to interact with lipid bilayer membranes. We observed histamine release by magainin-2 amide from rat peritoneal mast cells (ED50 = 13 micrograms/ml) but not from human basophils. This histamine-releasing reaction from peritoneal mast cells was due to a secretory rather than cytolytic effect, i.e., release occurred without concomitant liberation of lactic dehydrogenase. Furthermore, the pretreatment of mast cells with magainin-2 amide did not desensitize cells against subsequent challenge with other secretagogues. Maximum histamine release occurred in less than a minute at 25 and 37 degrees C. The addition of Ca2+ was not required for histamine release, although release was enhanced by the addition of 0.3-1 mM Ca2+. The addition of 3 mM Ca2+ or Mg2+ was markedly inhibitory. The presence of Na+ or Cl- ions in the medium was not required for release. Therefore, histamine release is not due to the formation of anion-selective channels in the membrane of mast cells. The results indicated that the characteristics of histamine secretion induced by magainin-2 amide were unlike IgE-mediated release but were similar to the mechanism of release attributed to some other basic peptides and to compound 48/80. Topics: Amino Acid Sequence; Animals; Anti-Infective Agents; Antimicrobial Cationic Peptides; Calcium; Cell Line; Histamine Release; In Vitro Techniques; Kinetics; L-Lactate Dehydrogenase; Leukemia, Basophilic, Acute; Magainins; Magnesium; Male; Mast Cells; Molecular Sequence Data; Peptides; Rats; Rats, Inbred Strains; Temperature; Xenopus Proteins | 1990 |