lys01 and Melanoma

Autophagy mediates resistance to various anticancer agents. In melanoma, resistance to targeted therapy has been linked to expression of Wnt5A, an intrinsic inhibitor of β-catenin, which also promotes invasion. In this study, we assessed the interplay between Wnt5A and autophagy by combining expression studies in human clinical biopsies with functional analyses in cell lines and mouse models. Melanoma cells with high Wnt5A and low β-catenin displayed increased basal autophagy. Genetic blockade of autophagy revealed an unexpected feedback loop whereby knocking down the autophagy factor ATG5 in Wnt5A

Topics: Aminoquinolines; Animals; Autophagy; Autophagy-Related Protein 5; beta Catenin; Blotting, Western; Cell Line, Tumor; Feedback, Physiological; Gene Expression Regulation, Neoplastic; Humans; Melanoma; Mice; Polyamines; Reverse Transcriptase Polymerase Chain Reaction; RNA Interference; Wnt Signaling Pathway; Wnt-5a Protein