lucifer-yellow and Choriocarcinoma

lucifer-yellow has been researched along with Choriocarcinoma* in 1 studies

Other Studies

1 other study(ies) available for lucifer-yellow and Choriocarcinoma

ArticleYear
Regulation of connexin31 gene expression upon retinoic acid treatment in rat choriocarcinoma cells.
    Experimental cell research, 1996, Aug-25, Volume: 227, Issue:1

    The controlled invasiveness of the trophoblast is based on the balance between invasive properties at implantation and the differentiation program of the developing placenta. During placental development in rats a switch of connexin gene expression has been observed in parallel to the switch from the invasive to the differentiated phenotype of trophoblast cells. To investigate the role of connexin expression for trophoblast invasion, proliferation, and differentiation, we studied one rat trophoblast (HRP-1) and one rat choriocarcinoma cell line (Rcho-1). The choriocarcinoma cells were characterized by expression of cx31 and a lack of E-cadherin, corresponding to the invasive trophoblast in vivo, whereas HRP-1 cells expressed cx43, normally found in the spongiotrophoblast and in late giant cells, and E-cadherin. Upon retinoic acid treatment, Rcho-1 cells irreversibly lost cx31 expression, accompanied by a loss of functional coupling. No changes in regard to connexin expression and cell-cell communication could be observed in HRP-1 cells. In addition, treatment of Rcho-1 cells with retinoic acid for 7 days upregulated expression of cx43 transcript, but no protein could be found. Proliferation was clearly reduced and the mean volume of cells doubled from Day 4 to Day 7 of retinoic acid treatment in Rcho-1 cells, while both parameters were not affected in HRP-1 cells. Both cell lines showed a similar invasion rate using a Matrigel invasion assay, and invasion was equally suppressed upon retinoic acid treatment. Thus the different connexin expression appears more likely to play a role in regulating proliferation and differentiation along the multilineage pathway than invasiveness of rat trophoblast cells.

    Topics: Animals; Cadherins; Calcium; Cell Communication; Cell Division; Cell Movement; Choriocarcinoma; Collagen; Connexin 43; Connexins; Drug Combinations; Fluorescent Dyes; Gap Junctions; Gene Expression Regulation, Developmental; Gene Expression Regulation, Neoplastic; Isoquinolines; Laminin; Proteoglycans; Rats; RNA, Messenger; Tretinoin; Trophoblasts; Tumor Cells, Cultured

1996