lucensomycin has been researched along with Hemolysis* in 3 studies
3 other study(ies) available for lucensomycin and Hemolysis
| Article | Year |
|---|---|
Protoporphyrin IX sensitized photohemolysis: stoichiometry of the reaction and repair by reduced glutathione.
Protoporphyrin IX acts as a sensitizer in the photohemolysis of bovine erythrocytes by binding to a limited number of membrane sites. The cholesterol-specific antibiotic lucensomycin competes with protoporphyrin in binding to the membranes. The possibility of cholesterol peroxidation as a primary event in photohemolysis is supported by the repairing effect of exogenous cholesterol and by the increased susceptibility of the photosensitized erythrocytes to lucensomycin. Glutathione, if present within the erythrocyte, postpones the onset of lysis; if added after irradiation, it may repair the membrane damage and prevent hemolysis. This effect appears to be related to a redox reaction (possibly involving glutathione peroxidase) between reduced glutathione and the cholesterol peroxide molecules. Topics: Animals; Cattle; Cholesterol; Dose-Response Relationship, Drug; Dose-Response Relationship, Radiation; Glutathione; Hemolysis; Light; Lucensomycin; Membrane Lipids; Oxidation-Reduction; Peroxides; Photolysis; Porphyrins; Protoporphyrins | 1977 |
Stoichiometry of hemolysis by the polyene antibiotic lucensomycin.
The stoichiometry of hemolysis by the polyene antibiotic lucensomycin was investigated. It appears that hemolysis occurs only when a relatively high fraction (probably between 15 and 40%) of the cholesterol sites in the erythrocyte membrane have combined with the polyene. Also in phospholipid-cholesterol vesicles the increase of permeability requires occupancy of 40-50% of the existing cholesterol sites. As for the possible cooperative effect in the hemolytic process, it is probable that several (at least 9-10) lucensomycin-cholesterol adducts must interact on each side of the membrane to form an aqueous channel; the distribution of these adducts in the erythrocyte membrane occurs, however, apparently at random. Topics: Animals; Antifungal Agents; Binding Sites; Cattle; Cholesterol; Erythrocytes; Hemolysis; Kinetics; Lucensomycin; Mathematics; Membrane Lipids; Membranes, Artificial; Models, Biological | 1976 |
Concentration of "available" unesterified cholesterol in human plasma as evaluated from inhibition of hemolysis by lucensomycin.
The unesterified cholesterol content of plasma samples can be evaluated from the extent of inhibition of lucensomycin-induced hemolysis. The test measures, however, only the fraction of cholesterol which is available for interaction with lucensomycin, this availability being adversely affected by high phospholipid-cholesterol ratios. Topics: Animals; Antifungal Agents; Cattle; Cholesterol; Cholesterol Esters; Digitonin; Erythrocytes; Hemolysis; Humans; Liposomes; Lucensomycin; Phosphatidylcholines; Phospholipids | 1976 |