losartan-potassium and Nephritis

Topics: Anemia; Animals; Antimicrobial Cationic Peptides; Erythropoietin; Hepcidins; Humans; Mice; Mononuclear Phagocyte System; Nephritis

Topics: 6-Ketoprostaglandin F1 alpha; China; Drugs, Chinese Herbal; Erythropoietin; Humans; Kidney; Nephritis; Nephrology

Topics: Aminolevulinic Acid; Anemia; Animals; Bone Marrow; Bone Marrow Cells; Depression, Chemical; Erythrocytes; Erythropoiesis; Erythropoietin; Estrogens; Feedback; Female; Heme; Hemoglobins; Humans; Hypoxia; Immunoglobulin G; In Vitro Techniques; Infant, Newborn; Iron; Male; Mice; Models, Biological; Nephritis; Polycythemia; Polycythemia Vera; Rats

Plasma erythropoietin levels were determined by Keighley's method to study the changes in erythropoiesis before and after human renal homotransplantation. Results. Erythropoietin titres in pre-transplant patients were low, while they returned to normal after successful renal transplantation. In acute rejection they were significantly high. After its reversal normal levels of erythropoietin were obtained in accordance with a normalization of the graft functions. Reticulocyte counts paralleled with erythropoietin values. Conclusion. High levels of plasma erythropoietin contribute to the diagnosis of acute rejection. Normalization of the plasma erythropoietin levels after the acute rejection could be regarded as an indication for good function of the graft. The grafted kidneys seem to function in producing erythropoietin.

Topics: Adult; Aminohippuric Acids; Blood Urea Nitrogen; Clinical Trials as Topic; Creatinine; Erythropoietin; Female; Graft Rejection; Hemoglobins; Humans; Kidney Transplantation; Male; Nephritis; Postoperative Complications; Reticulocytes; Transplantation, Autologous; Tuberculosis, Renal; Ureteral Obstruction

An increasing number of experiments and clinical trials have demonstrated the safety, feasibility, and efficacy of mesenchymal stem cells (MSCs)-based therapies for the treatment of various diseases. The main drawbacks of MSC therapy are the lack of specific homing after systemic infusion and early death of injected cells because of the injury micro-environment. We pretreated bone mesenchymal stem cells (BMSCs) with erythropoietin (EPO) to investigate their positive effect on cyclosporine A (CsA)-induced nephrotoxicity. BMSCs were incubated with different concentrations of EPO (10, 100, 500, and 1000 IU/mL) for 24 and 48 h, and their proliferation rate, cytoskeletal morphology, migration ability, and the expression of CXCR4 were evaluated to determine the optimal pretreatment conditions. To investigate the therapeutic effects of BMSCs pretreated with EPO in CsA-induced nephrotoxicity, we established CsA-induced in vitro and in vivo toxicity models. In our in vitro study, preconditioning of BMSCs with 500 IU/mL EPO for 48 h induced a marked increase in their proliferation rate, cytoskeletal rearrangement, migration in the scrape-healing assay, and migration toward injured HK2 cells. In vivo, EPO-BMSCs showed higher ability to improve renal function than BMSCs, and in CsA-induced rats treated with EPO-BMSCs, interstitial lymphocyte infiltration, tubular swelling, necrosis, and interstitial fibrosis decreased. We demonstrated that pretreatment with 500 IU/mL EPO before infusion markedly increased the homing ability of BMSCs, and obviously ameliorate CsA-induced nephrotoxicity in rats.

Topics: Animals; Bone Marrow Cells; Cell Differentiation; Cell Line; Cell Movement; Cell Proliferation; Coculture Techniques; Cyclosporine; Epithelial Cells; Erythropoietin; Female; Gene Expression; Graft Survival; Kidney; Kidney Function Tests; Mesenchymal Stem Cell Transplantation; Mesenchymal Stem Cells; Nephritis; Primary Cell Culture; Rats; Rats, Sprague-Dawley; Receptors, CXCR4

Erythropoietin (EPO) is a potent stimulator of erythroid progenitor cells and is known to be up-regulated during states of hypoxia. Here we investigate the effects of renal ischemia/reperfusion (I/R) on the degree of renal dysfunction and injury with recombinant human EPO in mice when given as either a 3-day pretreatment, or upon reperfusion of the kidney.. Mice were treated with EPO (1000 IU/kg/day subcutaneously) for 3 days, or treated with EPO (1000 IU/kg subcutaneously) upon reperfusion, and subsequently subjected to bilateral renal artery occlusion (30 minutes) and reperfusion (24 hours). At the end of experiments, the following indicators and markers of renal injury and dysfunction were measured: plasma urea, creatinine, and aspartate aminotransferase (AST), tissue myeloperoxidase (MPO) activity [for polymorphonuclear leukocyte (PMN) infiltration], and tissue malonaldehyde (MDA) levels (for tissue lipid peroxidation). Kidneys were used for histologic evaluation of renal injury.. EPO was able to significantly attenuate the renal dysfunction and injury associated with I/R, as well as the tissue injury. The increase in renal MPO activity and, hence, the degree of PMN infiltration were also significantly reduced in EPO-treated mice. In addition, lipid peroxidation as a result of renal I/R injury was also attenuated in EPO-treated mice.. The protection afforded by the pretreatment regime of EPO was greater than that of administering EPO as a single bolus upon reperfusion. We propose that different mechanisms underlie the protective effects seen with EPO when given as either a daily pretreatment or as a single bolus, which need to be further investigated.

Topics: Animals; Aspartate Aminotransferases; Creatinine; Erythropoietin; Male; Mice; Mice, Inbred C57BL; Nephritis; Reperfusion Injury; Urea

The stimulatory effect of spa therapy on erythropoiesis is well documented. The present study aimed to elucidate the pathogenesis of this effect. The influence of spa therapy on plasma erythropoietin and erythropoiesis was studied in four groups of patients: 35 patients with essential hypertension, 35 patients with inflammatory renal diseases at a stabilized stage and normal excretory renal function. 25 patients with gastrointestinal pathology or cholelithiasis and 33 patients with neurovegetative neurosis. Spa therapy for 20 days in Wysowa was accompanied by a significant increase of plasma erythropoietin, iron, ferritin and saturation of transferrin with iron and by an increase of blood haemoglobin and haematocrit value. These alterations were especially marked in patients with essential hypertension.. 1. Spa therapy exerts a stimulatory effect on erythropoiesis caused, among other factors, by increased erythropoietin secretion and iron mobilization. 2. This stimulatory effect is especially marked in patients with essential hypertension.

Topics: Autonomic Nervous System Diseases; Balneology; Circadian Rhythm; Erythropoiesis; Erythropoietin; Female; Ferritins; Gastrointestinal Diseases; Hematocrit; Hemoglobins; Humans; Hypertension; Iron; Male; Middle Aged; Nephritis

Topics: Anemia; Erythropoietin; Female; Humans; Kidney Failure, Chronic; Male; Middle Aged; Nephritis; Renal Dialysis

Topics: Adult; Anemia; Autonomic Nervous System; Blood Pressure; Dopamine beta-Hydroxylase; Erythropoietin; Female; Glomerulonephritis; Humans; Male; Middle Aged; Nephritis; Norepinephrine; Pre-Eclampsia; Pregnancy; Recombinant Proteins; Renal Dialysis

Topics: Adolescent; Child; Child, Preschool; Chronic Disease; Erythropoietin; Humans; Nephritis

Topics: Anemia; Animals; Biliary Fistula; Constriction; Erythropoietin; Female; Humans; Iron Isotopes; Kidney; Liver; Liver Cirrhosis; Nephritis; Phenylhydrazines; Rats; Spleen; Trypan Blue