losartan-potassium and Escherichia-coli-Infections

The anemia of inflammation is related in part to abnormal erythropoiesis in bone marrow. G-CSF regulates granulopoiesis and is increased during systemic inflammation. Here, we have showed that high levels of G-CSF are associated with repression of bone marrow erythropoiesis and expansion of splenic erythropoiesis in

Topics: Animals; Bone Marrow; Erythropoiesis; Erythropoietin; Escherichia coli; Escherichia coli Infections; Granulocyte Colony-Stimulating Factor; Injections, Subcutaneous; Lipopolysaccharides; Mice; Mice, Inbred C57BL; Mice, Knockout; Signal Transduction; Spleen; Systemic Inflammatory Response Syndrome; Toll-Like Receptor 4

Endogenous mechanisms underlying bacterial infection resolution are essential for the development of novel therapies for the treatment of inflammation caused by infection without unwanted side effects. Herein, we found that erythropoietin (EPO) promoted the resolution and enhanced antibiotic actions in

Topics: Animals; Anti-Bacterial Agents; Disease Models, Animal; Disease Susceptibility; Drug Resistance, Bacterial; Erythropoietin; Escherichia coli; Escherichia coli Infections; Host-Pathogen Interactions; Macrophages; Mice; Peritonitis; Phagocytosis; PPAR gamma; Signal Transduction; Staphylococcal Infections; Staphylococcus aureus

To investigate the possible ameliorating effect of recombinant human erythropoietin (rhEPO) on white matter damage, pro-inflammatory cytokine and chemokine induction in developing rat brain after intra-uterine Escherichia coli infection. E. coli was inoculated into uterine cervix of the time-pregnant rats and the control was injected with normal saline. Following maternal E. coli inoculation, the pups received a single intraperitoneal injection of rhEPO at a dose of 5000 IU/kg body weight immediately after birth. Immunohistochemical staining and Western blot analysis for 2', 3'-cyclic nucleotide 3'-phosphodiesterase (CNPase), neurofilament (NF) and glial fibrillary acidic protein (GFAP) were performed to assess white matter damage in pup brains at post-natal day 1 (P1), P3 and P7. Pro-inflammatory cytokines and chemokines were detected by real-time quantitative RT-PCR at the mRNA levels to evaluate the inflammatory response in pup brains at P1, P3 and P7. A single dose of rhEPO treatment (5000 IU/kg body weight) attenuated white matter damage in developing rat brain after intra-uterine E. coli infection. The protein levels of CNPase and NF in pup brains at P7 significantly increased after post-natal rhEPO treatment as compared with the intra-uterine E. coli-treated group. Also, post-natal rhEPO injection markedly attenuated the intra-uterine E. coli infection-induced increases in GFAP protein expression and the mRNA levels of pro-inflammatory cytokines and chemokines. Post-natal EPO administration as a single dose may exert a neuroprotective effect on white matter damage by reducing pro-inflammatory cytokine and chemokine induction in developing rat brain after intra-uterine E. coli infection.

Topics: Animals; Animals, Newborn; Brain; Chemokines; Cytokines; Erythropoietin; Escherichia coli Infections; Female; Fetal Diseases; Humans; Nerve Fibers, Myelinated; Neuroprotective Agents; Pelvic Infection; Pregnancy; Pregnancy Complications, Infectious; Random Allocation; Rats; Rats, Sprague-Dawley; Recombinant Proteins; Time Factors

Topics: Anemia; Animals; Bone Marrow Examination; Erythrocyte Count; Erythropoiesis; Erythropoietin; Escherichia coli Infections; Hematocrit; Hemoglobinometry; Pyelonephritis; Rats; Reticulocytes; Stimulation, Chemical