lithium-chloride and Uveitis

To evaluate the anti-inflammatory effect of lithium chloride in endotoxin-induced uveitis.. A total of 200 Wistar rats were randomly divided into four groups: a control group; EIU group; LiCl-treated control group; and LiCl-treated lipopolysaccharide group. Clinical score, slit-lamp photography, hematoxylin and eosin (H&E) staining were used to determine the degree of inflammatory reaction. Level of glycogen synthase kinase3-beta and nuclear factor-kappa B p65 in iris-ciliary body was examined by western blot and RT-PCR. Cytokines in aqueous humor were detected by ELISA.. Pretreatment with LiCl produced an anti-inflammatory effect during endotoxin-induced uveitis (EIU). With LiCl treatment, the level of P-GSK3-β in iris-ciliary body was upregulated and the expression of NF-κB p65 was significantly suppressed during EIU.. LiCl pretreatment can suppress intraocular inflammatory responses in EIU rats. Mechanistically, this anti-inflammatory effect may be related to the inhibitory phosphorylation of GSK3-β.

Topics: Adjuvants, Immunologic; Animals; Aqueous Humor; Blotting, Western; Carrier Proteins; Ciliary Body; Disease Models, Animal; Enzyme-Linked Immunosorbent Assay; Gene Expression Regulation; Intracellular Signaling Peptides and Proteins; Iris; Lithium Chloride; Male; Rats; Rats, Wistar; Real-Time Polymerase Chain Reaction; Transcription Factor RelA; Tumor Necrosis Factor-alpha; Uveitis