liproxstatin-1 and Coronavirus-Infections

liproxstatin-1 has been researched along with Coronavirus-Infections* in 1 studies

Other Studies

1 other study(ies) available for liproxstatin-1 and Coronavirus-Infections

ArticleYear
Inhibiting ACSL1-Related Ferroptosis Restrains Murine Coronavirus Infection.
    Viruses, 2021, 11-28, Volume: 13, Issue:12

    Murine hepatitis virus strain A59 (MHV-A59) was shown to induce pyroptosis, apoptosis, and necroptosis of infected cells, especially in the murine macrophages. However, whether ferroptosis, a recently identified form of lytic cell death, was involved in the pathogenicity of MHV-A59 is unknown. We utilized murine macrophages and a C57BL/6 mice intranasal infection model to address this. In primary macrophages, the ferroptosis inhibitor inhibited viral propagation, inflammatory cytokines released, and cell syncytia formed after MHV-A59 infection. In the mouse model, we found that in vivo administration of liproxstatin-1 ameliorated lung inflammation and tissue injuries caused by MHV-A59 infection. To find how MHV-A59 infection influenced the expression of ferroptosis-related genes, we performed RNA-seq in primary macrophages and found that MHV-A59 infection upregulates the expression of the acyl-CoA synthetase long-chain family member 1 (ACSL1), a novel ferroptosis inducer. Using ferroptosis inhibitors and a TLR4 inhibitor, we showed that MHV-A59 resulted in the NF-kB-dependent, TLR4-independent ACSL1 upregulation. Accordingly, ACSL1 inhibitor Triacsin C suppressed MHV-A59-infection-induced syncytia formation and viral propagation in primary macrophages. Collectively, our study indicates that ferroptosis inhibition protects hosts from MHV-A59 infection. Targeting ferroptosis may serve as a potential treatment approach for dealing with hyper-inflammation induced by coronavirus infection.

    Topics: Animals; Coenzyme A Ligases; Coronavirus Infections; Cytokines; Disease Models, Animal; Ferroptosis; Genes, Viral; Lung Injury; Macrophages; Mice; Mice, Inbred C57BL; Murine hepatitis virus; Quinoxalines; RAW 264.7 Cells; Spiro Compounds; Toll-Like Receptor 4; Virus Replication

2021