lipoxin-a4-methyl-ester and Cerebrovascular-Disorders

Since autophagy and endoplasmic reticulum stress mechanisms are involved in some neurodegenerative and cerebral vascular diseases, we suspected that similar mechanisms might participate in vascular cognitive impairments induced by chronic cerebral hypoperfusion. Lipoxin A4 methyl ester (LXA4 ME) is an inflammation inhibitor that exhibits potent protective effects in experimental stroke models. In an earlier study, we found that LXA4 ME improved cognitive deficit in a rat model of vascular cognitive impairment created using bilateral common carotid artery ligation (BCCAL) and two-vessel occlusion (2VO). In this study, LXA4 ME treatment of 2VO rats improved brain morphological defects. We found that LXA4 ME reduced the expression of some autophagy- and ERS-related factors in the hippocampus of 2VO rats, namely C/EBP homologous protein, beclin1 and the ratio of microtubule-associated protein light chain 3 II (LC3-II) to LC3-I. By contrast, LXA4 ME upregulated the protein expression of phospho-mTOR, total-mTOR, glucose-regulated protein 78 and spliced and unspliced X-box binding protein-1 mRNA. Differential protein regulation by LXA4 ME might underlie its ability to protect cognition after chronic cerebral hypoperfusion.

Topics: Animals; Apoptosis Regulatory Proteins; Autophagy; Beclin-1; Blotting, Western; Carotid Artery, Common; Cerebrovascular Disorders; Cognition Disorders; DNA-Binding Proteins; Endoplasmic Reticulum Stress; Gene Expression; Hippocampus; Lipoxins; Male; Microtubule-Associated Proteins; Rats, Sprague-Dawley; Regulatory Factor X Transcription Factors; Reverse Transcriptase Polymerase Chain Reaction; TOR Serine-Threonine Kinases; Transcription Factors