lipofectamine and Peritoneal-Neoplasms

lipofectamine has been researched along with Peritoneal-Neoplasms* in 1 studies

Other Studies

1 other study(ies) available for lipofectamine and Peritoneal-Neoplasms

ArticleYear
High-Pressure Nebulization as Application Route for the Peritoneal Administration of siRNA Complexes.
    Macromolecular bioscience, 2017, Volume: 17, Issue:10

    Peritoneal carcinomatosis is a severe form of cancer in the abdomen, currently treated with cytoreductive surgery and intravenous chemotherapy. Recently, nebulization has been proposed as a less invasive strategy for the local delivery of chemotherapeutic drugs. Also, RNA interference has been considered as a potential therapeutic approach for treatment of cancer. In this study, Lipofectamine RNAiMAX/siRNA complexes and cyclodextrin/siRNA complexes are evaluated before and after nebulization. Nebulization of the siRNA complexes does not significantly lower transfection efficiency when compared to non-nebulized complexes. After incubation in ascites fluid, however, the cyclodextrin/siRNA complexes show a drastic decrease in transfection efficiency. For the Lipofectamine RNAiMAX/siRNA complexes, this decrease is less pronounced. It is concluded that nebulization is an interesting technique to distribute siRNA complexes into the peritoneal cavity, providing the complexes are stable in ascites fluid which might be present in the peritoneal cavity.

    Topics: Aerosols; Ascites; Cell Line, Tumor; Cyclodextrins; Female; Humans; Lipids; Luciferases; Ovarian Neoplasms; Peritoneal Neoplasms; Plasmids; RNA, Small Interfering; Transfection

2017