lipofectamine and Lymphoma--Large-B-Cell--Diffuse

To characterize the effect of human immunodeficiency virus-1 (HIV-1) nef expression in human monocytes/macrophage (HMO) and U937 on the levels of FcgammaRs, HLA antigens, and monokines, elutriated HMOs and U937 cells were transfected with an adenovirus-mediated Nef expression system. Nef-expressing cells downmodulated FcgammaRI, FcgammaRII, and upregulated HLA class I molecules. Nef-expressing HMOs, treated with lipopolysaccharide (LPS) or phorbol 12-myristate 13-acetate (PMA), overexpressed tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), and IL-10. However, IL-6 was induced by LPS and inhibited by PMA. Additionally, a subpopulation of Nef-expressing HMOs underwent apoptosis. Our data suggest that HIV-1 nef downmodulated FcgammaRs in myeloid cells in a manner similar to that previously reported for its effect on CD4+ in T cells.

Topics: 3T3 Cells; Adenoviridae; Animals; Apoptosis; Cation Exchange Resins; CD4 Antigens; DNA, Antisense; Down-Regulation; Gene Expression Regulation, Viral; Gene Products, nef; Genes, nef; Genetic Vectors; HIV-1; HLA Antigens; Humans; Interleukin-1; Interleukin-10; Interleukin-6; Lipids; Lipopolysaccharides; Lymphoma, Large B-Cell, Diffuse; Macrophages; Mice; Monocytes; Monokines; nef Gene Products, Human Immunodeficiency Virus; Receptors, IgG; Receptors, Interleukin-2; Tetradecanoylphorbol Acetate; Transfection; Tumor Cells, Cultured; Tumor Necrosis Factor-alpha