lipid-a and Thrombosis

Topics: Animals; Arteriosclerosis; Blood Circulation; Blood Platelets; Blood Vessels; Cells, Cultured; Collagen; Endothelium; Hemostasis; Humans; Hyaluronoglucosaminidase; In Vitro Techniques; Lipid A; Microscopy, Electron, Scanning; Models, Biological; Neuraminidase; Prostaglandins; Rabbits; Thrombin; Thrombosis; von Willebrand Diseases

To investigate the crossreactivity of anti-cardiolipin antibodies (aCL) with glycolipids and endothelial cells, we used (1) culture supernatants derived from Epstein-Barr virus (EBV)-transformed peripheral lymphocytes of patients with systemic lupus erythematosus (SLE) complicated by antiphospholipid syndrome, and also (2) sera from patients with SLE. The results obtained from culture supernatants demonstrated that aCL crossreacts with myelin-derived acidic glycolipid sulfatide, bacterial endotoxin glycolipid lipid A and human umbilical vein endothelial cells (HUVEC). The results obtained from sera showed a significant correlation between levels of aCL and anti-sulfatide antibody, and between levels of aCL and anti-HUVEC antibody. These findings indicate that aCL can bind to the myelin sheath and HUVEC, which may have implications for the pathogenic potential of aCL for nervous system involvement and thrombotic complications.

Topics: Antibodies, Anticardiolipin; Cells, Cultured; Cross Reactions; Endothelium, Vascular; Glycolipids; Humans; Lipid A; Lupus Erythematosus, Systemic; Thrombosis

Various reactions of disseminated intravascular coagulation (DIC) were experimentally induced by infusion of thrombokinase in rats, by administration of endotoxin in rabbits and pigs and by infusion of adrenaline and thrombin in dogs. Low plasma concentrations of recombinant hirudin (r-hirudin; 20-200 ng/ml) were sufficient for the inhibition of the different triggering mechanisms. The studies on the pharmacological profile of r-hirudin in DIC therapy confirm the efficacy of this specific tight-binding thrombin inhibitor.

Topics: Animals; Disease Models, Animal; Disseminated Intravascular Coagulation; Dogs; Endotoxins; Epinephrine; Factor Xa; Factor Xa Inhibitors; Female; Fibrinolytic Agents; Hemodynamics; Hirudin Therapy; Hirudins; Lipid A; Male; Platelet Count; Rabbits; Rats; Rats, Inbred Strains; Recombinant Proteins; Species Specificity; Swine; Thrombin; Thrombolytic Therapy; Thrombosis; Viscera