linoleic-acid and Hepatitis

In the Long-Evans Cinnamon rat, copper accumulates in the liver because of a mutation in the copper-transporting ATPase gene, and peroxidative stresses are supposed to be augmented. We examined the effects of dietary fatty acids on hepatitis, hepatic gene expression, and survival. Rats were fed a conventional, low-fat diet (CE2), a CE2 diet supplemented with 10 wt% of lard (Lar), high-linoleic soybean oil (Soy), or a mixture of docosahexaenoic acid (DHA)-rich fish oil and soybean oil (DHA/Soy). Among female rats, the mean survival times of the DHA/Soy and the Soy groups were longer by 17 approximately 20% than in the Lar and the CE2 groups. Among male rats, the survival times were much longer than in the females, but no significant difference in survival was observed among the dietary groups. Serum ceruloplasmin levels in female and male rats of all of the dietary groups were similar. Serum transaminase levels of the DHA/Soy group tended to be lower than in the CE2 group. Histological examinations revealed a marked degeneration in hepatic tissue integrity in the Lar and CE2 groups but not in the DHA/Soy group. Hepatic levels of metal-related genes, transferrin and ceruloplasmin, as well as those related to bile acid synthesis were up-regulated, and an inflammation-related gene (cyclooxygenase [COX]-2) was down-regulated in the DHA/Soy group. Some proliferation-related genes were also affected by the dietary fatty acids. These results indicate that polyunsaturated fatty acids suppress the development of acute hepatitis and prolong survival in females, regardless of whether they are of the n-6 or n-3 type, which are associated with altered gene expressions.

Topics: Acute Disease; Animals; Cyclooxygenase 1; Cyclooxygenase 2; Dietary Fats, Unsaturated; Disease Models, Animal; Docosahexaenoic Acids; Fatty Acids; Fatty Acids, Unsaturated; Female; Fish Oils; Gene Expression; Hepatitis; Hepatolenticular Degeneration; Isoenzymes; Linoleic Acid; Liver; Male; Membrane Proteins; Prostaglandin-Endoperoxide Synthases; Rats; Rats, Inbred LEC; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Soybean Oil; Survival Rate

Topics: Chemical and Drug Induced Liver Injury; Cholesterol; Chromatography; Fatty Acids; Fatty Acids, Essential; Fatty Liver; Geriatrics; Hepatitis; Hypervitaminosis A; Linoleic Acid; Lipid Metabolism; Lipids; Liver; Oleic Acid; Palmitic Acid; Phospholipids; Toxicology; Vitamin K

Topics: Biomedical Research; Carbon Tetrachloride Poisoning; Chemical and Drug Induced Liver Injury; Hepatitis; Hepatitis A; Linoleic Acid; Lipids; Liver Function Tests; Pharmacology; Rats

Topics: Amino Acids; Blood; Chemical and Drug Induced Liver Injury; Choline; Glycine max; Hepatitis; Linoleic Acid; Lipotropic Agents; Liver Cirrhosis; Liver Cirrhosis, Experimental; Liver Diseases; Phospholipids; Rats; Research; Sulfacetamide; Toxicology