linoleic-acid and Carbon-Tetrachloride-Poisoning

In the present study, in vitro antioxidant, antioxidative stress and hepatoprotective activity of Moringa oleifera Lam. seed oil (Ben oil; BO) was evaluated against carbon tetrachloride (CCl(4) ) induced lipid peroxidation and hepatic damage in rats. The oil at 0.2 and 0.4 mL/rat was administered orally for 21 consecutive days. The substantially elevated serum enzymatic (GOT, GPT, ALP, GGT) and bilirubin levels were significantly restored towards normalization by the oil. There was a significant elevation in the level of malondialdehyde (MDA), non-protein sulfhydryl (NP-SH), and total protein (TP) contents in the liver tissue. The results obtained indicated that BO possesses potent hepatoprotective action against CCl(4) -induced hepatic damage by lowering liver marker enzymes, MDA concentration, and elevating NP-SH and TP levels in liver tissue. The biochemical observations were supplemented with histopathological examination of rat liver. The results of this study showed that treatment with Ben oil or silymarin (as a reference) appears to enhance the recovery from hepatic damage induced by CCl(4) . The pentobarbital induced narcolepsy prolongation in mice was retarded by the Ben oil. Acute toxicity test in mice showed no morbidity or mortality. In vitro DPPH radical scavenging and β-carotene-linolic acid assay tests of the BO exhibited a moderate antioxidant activity in both tests used. The possible mechanism(s) of the liver protective activity of Ben oil activity may be due to free radical scavenging potential caused by the presence of antioxidant component(s) in the oil. Consequently, BO can be used as a therapeutic regime in treatment of some hepatic disorders.

Topics: Animals; Antioxidants; beta Carotene; Bilirubin; Biphenyl Compounds; Carbon Tetrachloride Poisoning; Chemical and Drug Induced Liver Injury; Female; Linoleic Acid; Lipid Peroxidation; Liver; Male; Malondialdehyde; Mice; Moringa oleifera; Picrates; Plant Oils; Rats; Rats, Wistar; Seeds; Silymarin

Erucic and alpha-linolenic acids are probably related to a damage of muscle. The research was defined to the question if CCl4 that causes lesions in the tissue of the rat) damages also the muscle and if the dietary unsaturated fatty acids are effective in the prevention. The alpha-linolenic acid causes a strong reduction of the docosaesaenoic acid in the muscle, while the reduction of arachidonic acid is quite similar in all studied organs.

Topics: Animals; Arachidonic Acid; Arachidonic Acids; Carbon Tetrachloride Poisoning; Dietary Fats; Erucic Acids; Linoleic Acid; Linoleic Acids; Male; Muscles; Rats; Rats, Inbred Strains

Damage of exocrine pancreas liver and lungs was induced by CCl4, probably because an unbalancement of essential fatty acids (EFA). The present research is made on the rats, injected with CCl4 subcutaneously and kept with diets at variable composition in linoleic acid, proceeding to the extraction of these organs for the gaschromatografic determination of fatty acids. CCl4 reduces the arachidonic acid in liver, pancreas and lungs of the injected rats and the dietary linoleic acid does not modify this picture in the organs.

Topics: Animals; Carbon Tetrachloride Poisoning; Fatty Acids, Essential; Linoleic Acid; Linoleic Acids; Liver; Lung; Male; Pancreas; Rats; Rats, Inbred Strains

The poly-unsaturated fatty acids of w-3 series were studied in the tissues of rats injected with CCl4 and also the effect of the alpha-linolenic acid added to the diet. Gascromathographic technique of fatty acids was applied on samples of liver, muscle, lungs, pancreas and adipose tissue. CCl4 was seen to increase in the liver and in the muscle the docosahexaenoic acid; the dietetic alpha-linolenic acid inhibits, in muscle, lungs and pancreas, the conversion of linoleic in arachidonic acid.

Topics: Adipose Tissue; Animals; Carbon Tetrachloride Poisoning; Linoleic Acid; Linoleic Acids; Linolenic Acids; Liver; Lung; Male; Muscles; Pancreas; Rats; Rats, Inbred Strains

Topics: Biomedical Research; Carbon Tetrachloride Poisoning; Chemical and Drug Induced Liver Injury; Hepatitis; Hepatitis A; Linoleic Acid; Lipids; Liver Function Tests; Pharmacology; Rats