linoleic-acid and Arthritis--Juvenile

To investigate the causal effects of plasma Polyunsaturated fatty acids (PUFAs) on the risk of juvenile idiopathic arthritis (JIA) and ocular comorbidity through Mendelian randomization (MR) analysis.. Genetic variants (formerly single nucleotide polymorphisms, SNPs) that are strongly associated with PUFAs levels (P < 5×10. In model 1, genetically predicted n-6 PUFAs linoleic acid (LA) and arachidonic acid (AA) were associated with lower and higher risk of JIA associated iridocyclitis using IVW (OR. Our MR study provides genetic evidence on the possible causality that plasma LA level might protect against JIA associated iridocyclitis, whereas AA was responsible for opposite effect.

Topics: Arachidonic Acid; Arthritis, Juvenile; Causality; Comorbidity; Fatty Acids, Unsaturated; Humans; Iridocyclitis; Linoleic Acid; Mendelian Randomization Analysis; Polymorphism, Single Nucleotide

The study objective was to determine the relationship, if any, between the levels of arachidonic and linoleic acids in erythrocyte membrane phosphatidylcholine (PCH), serum interleukin 6 (IL-6) and TNFalpha levels and C-reactive protein (CRP) in juvenile idiopathic arthritis (JIA).. The study involved 49 children with JIA, aged 3-18 years (mean 11.3 +/- 3.9), and 29 healthy subjects. The JIA children were divided into 2 groups: group I--24 children (in exacerbation period) with mean CRP level of 15.6 mg/L +/- 13.3 and group II--25 children (in remission period--joint swelling-free) with CRP of 7.8 mg/L +/- 5.8 on average. Lipids were extracted according to a modified Folch's method. Fatty acids in erythrocyte membrane PCH were identified using gas chromatography (Hewlett-Packard 5890). The levels of IL-6 and TNFalpha were determined by ELISA, using Quantikine sets: R&D System (USA), while CRP was measured by nephelometric method on a BN II apparatus (Behring).. We found a significant decrease in the level of linoleic acid (p < 0.05) and a statistically insignificant reduction in arachidonic acid in JIA patients as compared to the controls. The decrease in linoleic acid was more pronounced in the active phase of JIA (p < 0.001. The higher serum CRP level was accompanied by a significantly elevated level of IL 6 (p < 0.05). The concentration of TNFa was elevated, but the difference had no statistical significance.. The levels of linoleic and arachidonic acids in erythrocyte PCH decreased and the concentrations of IL-6 and TNF-alpha increased in JIA children in the active phase of the disease. The differences intensified with a rise in CRP.

Topics: Adolescent; Arachidonic Acids; Arthritis, Juvenile; Biomarkers; C-Reactive Protein; Child; Child, Preschool; Chromatography, Gas; Chromatography, Thin Layer; Enzyme-Linked Immunosorbent Assay; Erythrocyte Membrane; Female; Humans; Interleukin-6; Linoleic Acid; Male; Phosphatidylcholines; Tumor Necrosis Factor-alpha