ligustilide and Osteoporosis

Topics: 4-Butyrolactone; Angelica sinensis; Animals; Apoptosis; Bone Density Conservation Agents; Cell Line; Disease Models, Animal; Embryo, Nonmammalian; Gene Expression Regulation; Green Fluorescent Proteins; Larva; Mesenchymal Stem Cells; Mitogen-Activated Protein Kinase 1; Mitogen-Activated Protein Kinase 3; Osteoblasts; Osteoporosis; Prednisolone; Primary Cell Culture; Proto-Oncogene Proteins c-bcl-2; Rats; Receptors, G-Protein-Coupled; Zebrafish; Zebrafish Proteins

The purpose of the present study was to investigate the anti-inflammatory activity of Z-Ligustilide (LIG) in experimental ovariectomized (OVX) osteopenic rats. The anti-inflammatory potential of LIG in the regulation of nuclear factor kappa B (NF-κB), maleic dialdehyde (MDA), polymorphonuclear cells (PMN), interleukin-1β (IL-1β), inducible nitric oxide synthase (iNOS) and tumor necrosis factor-α (TNF-α), adhesion molecule (ICAM-1) and cyclooxygenase-2 (COX-2) was determined by ELISA. LIG significantly inhibited OVX-induced up-regulation of NF-κB activation and the production of IL-1β, TNF-α, iNOS, ICAM-1 and COX-2. Moreover, LIG suppressed MDA and infiltration of PMN. The results of the present study clearly demonstrate that there may be an inflammatory component in the etiology of osteoporosis. It revealed a significant anti-inflammatory effect of Z-Ligustilide in experimental OVX osteopenic rats.

Topics: 4-Butyrolactone; Aldehydes; Animals; Anti-Inflammatory Agents; Cyclooxygenase 2; Female; Inflammation; Intercellular Adhesion Molecule-1; Interleukin-1beta; Neutrophils; NF-kappa B; Nitric Oxide Synthase Type II; Osteoporosis; Ovariectomy; Rats; Rats, Wistar; Tumor Necrosis Factor-alpha; Up-Regulation