lignans and Prostatitis

To study the anti-inflammatory properties of OJ.. Ojayeonjonghwan (OJ) is a traditional Korean prescription, which has been widely used for the treatment of prostatitis. However, no scientific study has been performed of the anti-inflammatory effects of OJ.. Peritoneal macrophages were isolated 3-4 days after injecting a C57BL/6J mouse with thioglycollate. They were then treated with OJ water extract (0.01, 0.1, and 1 mg/mL) for 1 h and stimulated with lipopolysaccharide (LPS) for different times. Nitric oxide (NO), inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2, and proinflammatory cytokine levels were determined by NO assay, Western blotting, RT-PCR and ELISA.. OJ reduces inflammatory response, and this probably explains its positive impact on the prostatitis associated inflammation.

Topics: Animals; Anti-Inflammatory Agents, Non-Steroidal; Cells, Cultured; Cyclooctanes; Cyclooxygenase 2; Cytokines; Dioxoles; Ethnopharmacology; Gene Expression Regulation; Lignans; Lipopolysaccharides; Macrophage Activation; Macrophages, Peritoneal; Male; Medicine, Korean Traditional; Mice, Inbred C57BL; Nitric Oxide; Nitric Oxide Synthase Type II; Plant Extracts; Polycyclic Compounds; Prostatitis; Thioglycolates