lhrh--n-epsilon-azidobenzoyl-lys(6)- and Adenocarcinoma

lhrh--n-epsilon-azidobenzoyl-lys(6)- has been researched along with Adenocarcinoma* in 1 studies

Other Studies

1 other study(ies) available for lhrh--n-epsilon-azidobenzoyl-lys(6)- and Adenocarcinoma

Article	Year
Photoaffinity labelling of gonadotropin releasing hormone binding sites in human epithelial ovarian carcinomata. Biochemical and biophysical research communications, 1989, Jun-30, Volume: 161, Issue:3 A photoaffinity labelled derivative of [D-Lys6]-GnRH was prepared with a bifunctional photolabile reagent (4-azidobenzoyl)-N-hydroxysuccinimide. In rat pituitary membranes, this analog retained high binding affinity (Ka = 0.12 x 10(9) M-1) consistent with a single class of receptors. The analog was iodinated and used for the identification of GnRH binding sites in human epithelial ovarian carcinomata. By sodium dodecyl sulfate electrophoresis in 10% polyacrylamide gel the presence of two labelled components could be demonstrated: a high molecular weight component of 63,200 and a smaller component of 46,000. Competition experiments with unlabelled ligand suggest that it is the high molecular weight component which specifically binds GnRH. Topics: Adenocarcinoma; Affinity Labels; Azides; Carcinoma; Cell Membrane; Female; Gonadotropin-Releasing Hormone; Humans; Kinetics; Molecular Weight; Ovarian Neoplasms; Receptors, LHRH	1989

Article

Year

Photoaffinity labelling of gonadotropin releasing hormone binding sites in human epithelial ovarian carcinomata.

Biochemical and biophysical research communications, 1989, Jun-30, Volume: 161, Issue:3

A photoaffinity labelled derivative of [D-Lys6]-GnRH was prepared with a bifunctional photolabile reagent (4-azidobenzoyl)-N-hydroxysuccinimide. In rat pituitary membranes, this analog retained high binding affinity (Ka = 0.12 x 10(9) M-1) consistent with a single class of receptors. The analog was iodinated and used for the identification of GnRH binding sites in human epithelial ovarian carcinomata. By sodium dodecyl sulfate electrophoresis in 10% polyacrylamide gel the presence of two labelled components could be demonstrated: a high molecular weight component of 63,200 and a smaller component of 46,000. Competition experiments with unlabelled ligand suggest that it is the high molecular weight component which specifically binds GnRH.

Topics: Adenocarcinoma; Affinity Labels; Azides; Carcinoma; Cell Membrane; Female; Gonadotropin-Releasing Hormone; Humans; Kinetics; Molecular Weight; Ovarian Neoplasms; Receptors, LHRH

1989