lewis-x-antigen and Mouth-Neoplasms

It has been reported in oral squamous cell carcinoma (OSCC) that myeloid-derived suppressor cells infiltrate tumor tissues. This study examined whether S-1 chemotherapy changes immune cell populations in the tumor microenvironment.. We examined 71 patients with of OSCC, including 51 patients who received preoperative S-1 chemotherapy. Immunohistochemistry for PD-L1, CD8, forkhead box protein 3 (FOXP3), and CD15 was performed using biopsy and resected specimens.. The numbers of CD8. Preoperative S-1 chemotherapy can potentially improve prognosis by reducing CD15

Topics: B7-H1 Antigen; Carcinoma, Squamous Cell; CD8-Positive T-Lymphocytes; Head and Neck Neoplasms; Humans; Lewis X Antigen; Lymphocytes, Tumor-Infiltrating; Mouth Neoplasms; Neoplasm Recurrence, Local; Prognosis; Tumor Microenvironment

Tumor-infiltrating lymphocytes (TILs) reportedly play a pivotal role in antitumor immunity against oral squamous cell carcinoma (OSCC); however, mechanisms governing TIL recruitment to OSCC tissues remain to be clarified. This study was undertaken to assess a potential association between TILs and high endothelial venule (HEV)-like vessels that express sialyl 6-sulfo Lewis X (LeX).. OSCC tissue sections (n=41) were subjected to immunohistochemistry for sialyl 6-sulfo LeX and CD34 to allow quantitation of HEV-like vessels. Triple immunohistochemistry for sialyl 6-sulfo LeX and either CD3 and CD20 or CD4 and CD8 was conducted to determine which lymphocyte subset is more closely associated with HEV-like vessels.. HEV-like vessels expressing sialyl 6-sulfo LeX were detected in 27 of 41 (65.9%) OSCC cases, and these vessels were more frequently found in early disease (T1/T2 stages) compared with advanced (T3/T4) stages. The number of T cells attached to the inner surface of these HEV-like vessels was significantly greater than that of B cells, while the number of CD4. Sialyl 6-sulfo LeX is displayed not only on HEV-like vessels but also on OSCC cells and may potentially function in antitumor immunity against OSCC.

Topics: Aged; Carcinoma, Squamous Cell; Cytotoxicity, Immunologic; Female; Humans; Immunohistochemistry; Lewis X Antigen; Lymphocytes, Tumor-Infiltrating; Male; Mouth Neoplasms; Oligosaccharides; Sialyl Lewis X Antigen

The present study aimed to determine significance of E-cadherin, a cell adhesion molecule, and sialyl Lewis-X (sLeX), a cell surface antigen, in oral carcinogenesis. Expressions of E-cadherin and sLeX were detected using western blot analysis from oral malignant (n=25), and oral precancerous tissues (OPC, n=20) and their adjacent normal tissues. An altered expression of E-cadherin (E-cad) and sLeX was observed in malignant and precancerous tissues. E-cad western blot revealed presence of two bands, a 120 kDa (native, E-cad120) and a 97 kDa (known as truncated E-cad97). The accumulation of truncated E-cad97 and sLeX in malignant and OPC tissues compared to their adjacent normal tissues was observed. Receiver's Operating Characteristics (ROC) curve analysis showed good discriminatory efficacy of E-cad97, E-cad97:120 ratio and sLeX between the malignant and adjacent. normal tissues. Further, a positive correlation of E-cad97 and sLeX overexpression with advanced stage of the disease and lymphnode metastasis was observed. The data suggest that E-cadherin truncation and sLeX overexpression are early events which may facilitate the tumor cells to metastasize. Also, overexpression E-cad97 and sLeX in OPC tissues may be useful to predict metastatic potentials of tumors at an early stage of oral carcinogenesis. Key words: Oral cancer, oral precancerous conditions, E-cadherin, sialyl Lewis-X, metastasis.

Topics: Adult; Antigens, Tumor-Associated, Carbohydrate; Biomarkers, Tumor; Blotting, Western; Cadherins; Carcinoma, Squamous Cell; Humans; Lewis X Antigen; Middle Aged; Mouth Neoplasms; Precancerous Conditions; Protein Processing, Post-Translational; ROC Curve; Sialyl Lewis X Antigen

In a previous study, we found tumor-associated tissue eosinophilia (TATE) to be a favourable prognostic indicator for oral squamous cell carcinomas. Special techniques such as autofluorescence or immunohistochemistry are reported to be sometimes necessary to detect the presence of intact and degranulating eosinophils within the tumors. The aim of this study was to compare the number of eosinophils identified routinely with hematoxylin and eosin stain and by immunohistochemistry in oral squamous cell carcinomas with TATE. Thirty specimens of oral squamous cell carcinoma of the tongue, floor of the mouth, retromolar area and inferior gingiva with TNM stages II and III were used for histopathological analysis. Three-micrometer sections were stained with hematoxylin and eosin and immunohistochemically with monoclonal anti-human granulocyte-associated antigen using a standard streptavidin-biotin-peroxidase complex technique. The number of eosinophils/mm2 in the invasive front of the tumors was automatically quantified in a x400 field using an image computer analyser. Univariate statistical analysis was carried out using Student's t test. The computer-assisted morphometric results showed that there was no statistically significant difference (p>0.05) in the number of eosinophils/mm2 identified by hematoxylin and eosin or immunostaining technique in oral squamous cell carcinomas with TATE. This result suggests that hematoxilyn and eosin routine stain is a useful technique for measuring eosinophils in squamous cell carcinoma with eosinophilic tumor infiltration.

Topics: Aged; Carcinoma, Squamous Cell; Eosinophilia; Eosinophils; Female; Humans; Immunohistochemistry; Lewis X Antigen; Male; Middle Aged; Mouth Neoplasms

Carbohydrate antigens in cancer cells are considered to be involved in the binding of cancer cells to the endothelium during metastasis.. Seventy cases of primary oral squamous cell carcinoma (SCC) were obtained from biopsy specimens and were analyzed immunohistochemically using an antibody against sialyl Lewis (Le)a or sialyl Le(x). Flow cytometry was performed to detect the sialyl Le(a) or sialyl Le(x) expressed on oral SCC cell lines.. The expressions of sialyl Le(a), but not sialyl Le(x), of primary tumors significantly correlated to nodal metastasis; 71% of the metastatic cases express sialyl Le(a) and the cases with positive sialyl Le(a) and no sialyl Le(x) demonstrated a high incidence of metastasis (80%). A flow cytometric study demonstrated the oral SCC cell line, which can metastasize in nude mice, to express a high level of sialyl Le(a).. The high expression of sialyl Le(a) in primary tumors may thus be involved in nodal metastasis and therefore predict a poor prognosis in oral SCC.

Topics: Animals; Antigens, Tumor-Associated, Carbohydrate; Biomarkers, Tumor; CA-19-9 Antigen; Carcinoma, Squamous Cell; E-Selectin; Flow Cytometry; Gangliosides; Humans; Immunohistochemistry; Lewis X Antigen; Lymphatic Metastasis; Male; Mice; Mice, Nude; Mouth Neoplasms; Oligosaccharides; Prognosis; Sialyl Lewis X Antigen; Tumor Cells, Cultured

Twenty-six biopsy specimens of oral squamous cell carcinomas were examined by the avidin biotin peroxidase complex (ABC) method for the presence of an epithelial cell membrane bound lacto-N-fucopentaose III, known also as Leu-M1 or Lex antigen. In normal oral epithelium, Leu-M1 antigen was expressed on keratinizing epithelia in the stratum spinosum. In well-differentiated carcinomas the antigen was found on the cell membrane of nucleate cells in infiltrating epithelial islands. Such pattern in moderately well and in poorly differentiated carcinomas was minimally expressed and was associated with flattened squamous cells or otherwise recorded negative. Leu-M1 antigen immunoreactivity in normal oral epithelia and in carcinomas was comparable to that of blood group H-2 chain that were examined. It was concluded that the intensity of the reaction parallels the magnitude of differentiation of epithelia. Leu-M1 antigen can serve as a marker of differentiation in oral squamous epithelium.

Topics: Animals; Antibodies, Neoplasm; Antigens, Differentiation, Myelomonocytic; Antigens, Neoplasm; Carcinoma, Squamous Cell; H-2 Antigens; Humans; Immunoenzyme Techniques; Lewis X Antigen; Mice; Mouth Neoplasms; Oligosaccharides