lewis-x-antigen and Hypereosinophilic-Syndrome

As the carbohydrate lacto-N-fucopentaose III (CD15 antigen or X-determinant) and its sialylated derivative sialyl-Lewis X are involved in the adhesion of cells rolling along the surface of endothelial cells, experiments were done to study the presence of these molecules on human eosinophils from patients with the idiopathic hypereosinophilic syndrome. Normal-density eosinophils from some patients showed higher levels of expression for lacto-N-fucopentaose III than light-density eosinophils. In contrast, sialyl-Lewis X was highly expressed by light-density eosinophils. Activation of normal-density eosinophils with calcium ionophore A23187 resulted in increased expression of these molecules for a short time. Monoclonal antibodies to these carbohydrates stimulated eosinophils to secrete eosinophil cationic protein, but not eosinophil peroxidase, and acted as costimulatory signals for C3b-induced degranulation of eosinophil cationic protein. It was suggested that CD15 and sialyl-Lewis X might contribute to eosinophil-mediated tissue injury in patients with the idiopathic hypereosinophilic syndrome.

Topics: Adult; Antibodies, Monoclonal; Blood Proteins; Calcimycin; Eosinophil Granule Proteins; Eosinophil Peroxidase; Eosinophils; Flow Cytometry; Humans; Hypereosinophilic Syndrome; Interleukin-5; Lewis X Antigen; N-Acetylneuraminic Acid; Peroxidase; Peroxidases; Respiratory Burst; Ribonucleases; Sialic Acids