lewis-x-antigen and Hermanski-Pudlak-Syndrome

lewis-x-antigen has been researched along with Hermanski-Pudlak-Syndrome* in 1 studies

Other Studies

1 other study(ies) available for lewis-x-antigen and Hermanski-Pudlak-Syndrome

Article	Year
Generation of Integration-Free Patient Specific iPS Cells Using Episomal Plasmids Under Feeder Free Conditions. Methods in molecular biology (Clifton, N.J.), 2016, Volume: 1353 Reprogramming somatic cells into a pluripotent state involves the overexpression of transcription factors leading to a series of changes that end in the formation of induced pluripotent stem cells (iPSCs). These iPSCs have a wide range of potential uses from drug testing and in vitro disease modelling to personalized cell therapies for patients. While viral methods for reprogramming factor delivery have been traditionally preferred due to their high efficiency, it is now possible to generate iPSCs using nonviral methods at similar efficiencies. We developed a robust reprogramming strategy that combines episomal plasmids and the use of commercially available animal free reagents that can be easily adapted for the GMP manufacture of clinical grade cells. Topics: Alkaline Phosphatase; Amides; Animals; Antigens, Surface; Cell Culture Techniques; Cell Differentiation; Cellular Reprogramming; Dermis; Fibroblasts; Gene Expression; Hermanski-Pudlak Syndrome; Humans; Induced Pluripotent Stem Cells; Intercellular Signaling Peptides and Proteins; Lewis X Antigen; Mice; Models, Biological; Octamer Transcription Factor-3; Plasmids; Pyridines; Serial Passage; Teratoma; Transgenes	2016

Article

Year

Generation of Integration-Free Patient Specific iPS Cells Using Episomal Plasmids Under Feeder Free Conditions.

Methods in molecular biology (Clifton, N.J.), 2016, Volume: 1353

Reprogramming somatic cells into a pluripotent state involves the overexpression of transcription factors leading to a series of changes that end in the formation of induced pluripotent stem cells (iPSCs). These iPSCs have a wide range of potential uses from drug testing and in vitro disease modelling to personalized cell therapies for patients. While viral methods for reprogramming factor delivery have been traditionally preferred due to their high efficiency, it is now possible to generate iPSCs using nonviral methods at similar efficiencies. We developed a robust reprogramming strategy that combines episomal plasmids and the use of commercially available animal free reagents that can be easily adapted for the GMP manufacture of clinical grade cells.

Topics: Alkaline Phosphatase; Amides; Animals; Antigens, Surface; Cell Culture Techniques; Cell Differentiation; Cellular Reprogramming; Dermis; Fibroblasts; Gene Expression; Hermanski-Pudlak Syndrome; Humans; Induced Pluripotent Stem Cells; Intercellular Signaling Peptides and Proteins; Lewis X Antigen; Mice; Models, Biological; Octamer Transcription Factor-3; Plasmids; Pyridines; Serial Passage; Teratoma; Transgenes

2016