lewis-x-antigen has been researched along with Gingivitis* in 2 studies
2 other study(ies) available for lewis-x-antigen and Gingivitis
Article | Year |
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Gingival crevicular neutrophils: membrane molecules do not distinguish between periodontitis and gingivitis.
We have earlier reported hyperreactive peripheral neutrophils in adult periodontitis, measured as respiratory burst after Fc gamma receptor-mediated activation in vitro, but we have not been able to relate this increased activity to aberrations in the expression of relevant membrane molecules. Various types of inflammatory conditions involving the gingiva should affect membranes differently. We therefore collected crevicular neutrophils from three types of inflammatory sites: (i) with and (ii) without tissue destruction in the same periodontitis patients and (iii) inflamed sites in controls with gingivitis alone and compared the expression of membrane molecules by flow cytometry. The % of positively stained cells and their mean intensities of fluorescence (IFL) were similar in the three types of sites for CD15, CD11a, CD11b and CD16. Peripheral neutrophils studied with the same markers were not activated. This was verified by similar plasma concentrations of lactoferrin and L-selectins in the periodontal and control groups. Compared to peripheral cells, the crevicular neutrophils showed a significantly lower percentage of stained cells, while the stained cells increased their IFL. In conclusion, hyperreactive peripheral neutrophils in periodontitis show the same expression of membrane molecules after migration through different types of inflammatory lesions as do normal neutrophils in gingivitis. Topics: Adult; Antigens, CD; CD11 Antigens; Cell Movement; Female; Flow Cytometry; Fluorescent Antibody Technique, Direct; Gene Expression Regulation; Gingival Crevicular Fluid; Gingivitis; Humans; L-Selectin; Lactoferrin; Lewis X Antigen; Male; Middle Aged; Neutrophil Activation; Neutrophils; Periodontitis; Receptors, IgG; Respiratory Burst | 1997 |
The ELAM-1 ligand sialosyl-Le(X) is present on Langerhans cells isolated from stratified epithelium.
In this study we show the expression of the newly identified carbohydrate ligand, sialosyl-Le(X) on Langerhans cells. The receptor for sialosyl-Le(X) is the endothelial leukocyte adhesion molecule-1 (ELAM-1) present on activated endothelial cells. Using flow cytometry, Langerhans cells were selected due to positivity for an antibody against CD1a and low orthogonal light scatter. The CD1a antigen stained by the OKT6 antibody is considered a maturational marker of Langerhans cells in agreement with the specific labeling of dendritic cells in the epithelium only. Double immunostaining (OKT6/anti-sialosyl-Le(X)) using flow cytometry and immunohistochemistry demonstrated that almost all OKT6-positive cells in normal stratified epithelium expressed sialosyl-Le(X). Conversely, by immunohistochemistry of oral epithelium with acute inflammation, additional dendritic cells negative for OKT6 were found to express sialosyl-Le(X). In addition, sialosyl-Le(X)-positive but not OKT6-positive dendritic cells were found in the submucosa. These findings indicate that the carbohydrate antigen sialosyl-Le(X) is expressed earlier than the CD1a antigen in the maturation of the Langerhans cell lineage. Future studies should aim at investigating the importance of adhesion between sialosyl Le(X) and ELAM-1 in epithelial recruitment of Langerhans cells. Topics: Antigens, CD; Antigens, CD1; Cell Adhesion Molecules; Cell Separation; Cheek; E-Selectin; Flow Cytometry; Fluorescent Antibody Technique; Gingivitis; Humans; Langerhans Cells; Lewis X Antigen; Ligands; Mouth Mucosa | 1992 |